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. 1977 Sep;6(3):187–194. doi: 10.1128/jcm.6.3.187-194.1977

Methyl mercaptan and dimethyl disulfide production from methionine by Proteus species detected by head-space gas-liquid chromatography.

N J Hayward, T H Jeavons, A J Nicholson, A G Thornton
PMCID: PMC274738  PMID: 332705

Abstract

Head-space gas-liquid chromatography and mass spectrometry were used to detect and identify products formed by Proteus vulgaris, P. mirabilis, P. morganii, and P. rettgeri from a defined medium supplemented with either phenylalanine, methionine, valine, leucine, histidine, lysine, ornithine, threonine, asparagine, aspartic acid, or tryptophan. In a detailed study of the products formed by 68 strains of Proteus spp. from L-methionine, the production of large amounts of both dimethyl disulfide and methyl mercaptan was found to be a characteristic of the genus. Both sulfur products appeared within a few hours of inoculation. Dimethyl disulfide was a more sensitive indicator of growth than the spectrometric determination of optical density. This suggests that it could be useful for the rapid, automated detection of any species of Proteus.

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Selected References

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  1. Birkinshaw J. H., Findlay W. P., Webb R. A. Biochemistry of the wood-rotting fungi: The production of methyl mercaptan by Schizophyllum commune Fr. Biochem J. 1942 Jun;36(5-6):526–529. doi: 10.1042/bj0360526. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Brooks J. B., Kellogg D. S., Alley C. C., Short H. B., Handsfield H. H., Huff B. Gas chromatography as a potential means of diagnosing arthritis. I. Differentiation between staphylococcal, streptococcal, gonococcal, and traumatic arthritis. J Infect Dis. 1974 Jun;129(6):660–668. doi: 10.1093/infdis/129.6.660. [DOI] [PubMed] [Google Scholar]
  3. CURRY A. S., HURST G., KENT N. R., POWELL H. Rapid screening of blood samples for volatile poisons by gas chromatography. Nature. 1962 Aug 11;195:603–604. doi: 10.1038/195603b0. [DOI] [PubMed] [Google Scholar]
  4. Carlsson J. Simplified gas chromatographic procedure for identification of bacterial metabolic products. Appl Microbiol. 1973 Feb;25(2):287–289. doi: 10.1128/am.25.2.287-289.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Cecchini G. L., O'Brien R. T. Detection of Escherichia coli by gas chromatography. J Bacteriol. 1968 Mar;95(3):1205–1206. doi: 10.1128/jb.95.3.1205-1206.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Drucker D. B. The identification of streptococci by gas-liquid chromatography. Microbios. 1972 Mar-Apr;5(18):109–112. [PubMed] [Google Scholar]
  7. MITSUHASHI S. Decomposition of thioether derivatives by bacteria; methylmercaptan formation and the properties of the responsible enzyme. Jpn J Exp Med. 1949 Sep;20(2):211–222. [PubMed] [Google Scholar]
  8. Mitruka B. M., Alexander M. Halogenated compounds for the sensitive detection of clostridia by gas chromatography. Can J Microbiol. 1972 Oct;18(10):1519–1523. doi: 10.1139/m72-232. [DOI] [PubMed] [Google Scholar]
  9. Mitruka B. M., Alexander M. Rapid and sensitive detection of bacteria by gas chromatography. Appl Microbiol. 1968 Apr;16(4):636–640. doi: 10.1128/am.16.4.636-640.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Mitruka B. M., Carmichael L. E., Alexander M. Gas chromatographic detection of in vitro and in vivo activities of certain canine viruses. J Infect Dis. 1969 Jun;119(6):625–634. doi: 10.1093/infdis/119.6.625. [DOI] [PubMed] [Google Scholar]
  11. Mitruka M., Kundargi R. S., Jonas A. M. Gas chromatography for rapid differentiation of bacterial infections in man. Med Res Eng. 1972;11(2):7–11. [PubMed] [Google Scholar]
  12. Moss C. W., Lambert M. A., Cherry W. B. Use of gas chromatography for determining catabolic products of arginine by bacteria. Appl Microbiol. 1972 May;23(5):889–893. doi: 10.1128/am.23.5.889-893.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. PROOM H., KNIGHT B. C. The minimal nutritional requirements of some species in the genus Bacillus. J Gen Microbiol. 1955 Dec;13(3):474–480. doi: 10.1099/00221287-13-3-474. [DOI] [PubMed] [Google Scholar]
  14. Reiner E., Hicks J. J., Ball M. M., Martin W. J. Rapid characterization of salmonella organisms by means of pyrolysis-gas-liquid chromatography. Anal Chem. 1972 May;44(6):1058–1061. doi: 10.1021/ac60314a031. [DOI] [PubMed] [Google Scholar]
  15. Ruiz-Herrera J., Starkey R. L. Dissimilation of methionine by a demethiolase of Aspergillus species. J Bacteriol. 1969 Sep;99(3):764–770. doi: 10.1128/jb.99.3.764-770.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Segal W., Starkey R. L. Microbial decomposition of methionine and identity of the resulting sulfur products. J Bacteriol. 1969 Jun;98(3):908–913. doi: 10.1128/jb.98.3.908-913.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Toan T. T., Bassette R., Claydon T. J. Methyl sulfide production by Aerobacter aerogenes in milk. J Dairy Sci. 1965 Sep;48(9):1174–1178. doi: 10.3168/jds.S0022-0302(65)88422-3. [DOI] [PubMed] [Google Scholar]
  18. WIESENDANGER S., NISMAN B. La Lméthionine démercapto-désaminase: un nouvel enzyme à pyridoxal-phosphate. C R Hebd Seances Acad Sci. 1953 Oct 5;237(14):764–765. [PubMed] [Google Scholar]
  19. Wade T. J., Mandle R. J. New gas chromatographic characterization procedure: preliminary studies on some Pseudomonas species. Appl Microbiol. 1974 Feb;27(2):303–311. doi: 10.1128/am.27.2.303-311.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Yoshioka, Kitamura M., Tamura Z. Rapid gas chromatographic analysis of microbial volatile metabolites. Jpn J Microbiol. 1969 Mar;13(1):87–93. doi: 10.1111/j.1348-0421.1969.tb00438.x. [DOI] [PubMed] [Google Scholar]

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