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. 1977 Sep;6(3):303–309. doi: 10.1128/jcm.6.3.303-309.1977

Enrichment medium for the isolation of Bordetella.

J Regan, F Lowe
PMCID: PMC274757  PMID: 198430

Abstract

The development of a specimen collection and transport medium outfit for the rapid laboratory diagnosis of whoping cough is described. The transport medium consisted of a semisolid agar containing charcoal, cephalexin, and defibrinated horse blood. It was also found to be an excellent enrichment medium for the selective isolation of Bordetella pertussis and B. parapertussis from scantily populated specimens. The investigation of 3,237 specimens that yielded 1,419 positive isolates of Bordetella, including 86 B. parapertussis, during a 20-month period is presented. A total of 3,076 specimens were processed in the laboratory by using the enrichment medium in addition to the routine procedure. Of these specimens, 757 were submitted in our medium, from which 137 (18%) were positive. Of the 567 specimens received in Amies transport medium, 290 (51%) positive cultures were obtained by the enrichment method only and not by primary culture.

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Selected References

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  1. Aftandelians R. V., Connor J. D. Bordetella pertussis serotypes in a whooping cough outbreak. Am J Epidemiol. 1974 May;99(5):343–346. doi: 10.1093/oxfordjournals.aje.a121619. [DOI] [PubMed] [Google Scholar]
  2. Amies C. R. A modified formula for the preparation of Stuart's Transport Medium. Can J Public Health. 1967 Jul;58(7):296–300. [PubMed] [Google Scholar]
  3. BRUMFITT W. Some growth requirements of Haemophilus influenzas and Haemophilus pertussis. J Pathol Bacteriol. 1959 Jan;77(1):95–100. doi: 10.1002/path.1700770109. [DOI] [PubMed] [Google Scholar]
  4. DONALDSON P., WHITAKER J. A. Diagnosis of pertussis by fluorescent antibody staining of nasopharyngeal smears. AMA J Dis Child. 1960 Apr;99:423–427. doi: 10.1001/archpedi.1960.02070030425004. [DOI] [PubMed] [Google Scholar]
  5. Diagnosis of whooping cough: comparison of serological tests with isolation of Bordetella pertussis. A combined Scottish study. Br Med J. 1970 Dec 12;4(5736):637–639. [PMC free article] [PubMed] [Google Scholar]
  6. ELDERING G., KENDRICK P. L. Incidence of parapertussis in the Grand Rapids area as indicated by 16 years' experience with diagnostic cultures. Am J Public Health Nations Health. 1952 Jan;42(1):27–31. doi: 10.2105/ajph.42.1.27. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Gästrin B., Kallings L. O., Marcetic A. The survival time for different bacteria in various transport media. Acta Pathol Microbiol Scand. 1968;74(3):371–380. doi: 10.1111/j.1699-0463.1968.tb03490.x. [DOI] [PubMed] [Google Scholar]
  8. HOLWERDA J., ELDERING G. CULTURE AND FLUORESCENT-ANTIBODY METHODS IN DIAGNOSIS OF WHOOPING COUGH. J Bacteriol. 1963 Sep;86:449–451. doi: 10.1128/jb.86.3.449-451.1963. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Holt L. B. The pathology and immunology of Bordetella pertussis infection. J Med Microbiol. 1972 Nov;5(4):407–424. doi: 10.1099/00222615-5-4-407. [DOI] [PubMed] [Google Scholar]
  10. Jamieson W. M. Whooping cough. Br Med J. 1973 Jan 27;1(5847):223–225. doi: 10.1136/bmj.1.5847.223. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Jones G. L., Kendrick P. L. Study of a blood-free medium for transport and growth of Bordetella pertussis. Health Lab Sci. 1969 Jan;6(1):40–45. [PubMed] [Google Scholar]
  12. KAUFMAN S., BRUYN H. B. Pertussis. A clinical study. AMA J Dis Child. 1960 Apr;99:417–422. [PubMed] [Google Scholar]
  13. Kendrick P. L., Gottshall R. Y., Anderson H. D., Volk V. K., Bunney W. E., Top F. H. Pertussis agglutinins in adults. Public Health Rep. 1969 Jan;84(1):9–15. [PMC free article] [PubMed] [Google Scholar]
  14. Kurt T. L., Yeager A. S., Guenette S., Dunlop S. Spread of pertussis by hospital staff. JAMA. 1972 Jul 17;221(3):264–267. [PubMed] [Google Scholar]
  15. Lautrop H. Epidemics of parapertussis. 20 years' observations in Denmark. Lancet. 1971 Jun 12;1(7711):1195–1198. doi: 10.1016/s0140-6736(71)91713-2. [DOI] [PubMed] [Google Scholar]
  16. Linnemann C. C., Jr, Bass J. W., Smith M. H. The carrier state in pertussis. Am J Epidemiol. 1968 Nov;88(3):422–427. doi: 10.1093/oxfordjournals.aje.a120903. [DOI] [PubMed] [Google Scholar]
  17. Linnemann C. C., Jr, Ramundo N., Perlstein P. H., Minton S. D., Englender G. S. Use of pertussis vaccine in an epidemic involving hospital staff. Lancet. 1975 Sep 20;2(7934):540–543. doi: 10.1016/s0140-6736(75)90907-1. [DOI] [PubMed] [Google Scholar]
  18. Morse S. I. Pertussis in adults. Ann Intern Med. 1968 Apr;68(4):953–954. doi: 10.7326/0003-4819-68-4-953. [DOI] [PubMed] [Google Scholar]
  19. Preston N. W. Technical problems in the laboratory diagnosis and prevention of whooping-cough. Lab Pract. 1970 May;19(5):482–486. [PubMed] [Google Scholar]
  20. Sutcliffe E. M., Abbott J. D. Selective medium for the isolation of bordetella pertussis and parapertussis. J Clin Pathol. 1972 Aug;25(8):732–733. doi: 10.1136/jcp.25.8.732. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. WHITAKER J. A., DONALDSON P., NELSON J. D. Diagnosis of pertussis by the fluorescent-antibody method. N Engl J Med. 1960 Oct 27;263:850–851. doi: 10.1056/NEJM196010272631708. [DOI] [PubMed] [Google Scholar]

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