Abstract
A head-space gas chromatographic technique for the analysis of volatile bacterial metabolites is described. Bacteroides fragilis, Clostridium perfringens, and Propionibacterium acnes, cultured in a glucose-containing peptone yeast extract medium, were studied. The head-space technique was compared with the injection of the complete liquid culture medium, and solvent extracts thereof, into the gas chromatograph. Volatile fatty acids could be detected by all three methods, whereas alcohols produced by C. perfringens and P. acnes were detectable only in the head-space chromatograms. Both FFAP and Porapak Q were used as gas chromatography stationary phase. Porapak Q was found more suitable than FFAP for the separation of alcohols. The head-space technique requires a minimum of preparation before the analysis and is well suited to automation.
Full text
PDF
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Bawdon R. E., Bassette R. Differentiation of Escherichia coli and Aerobacter aerogenes by gas liquid chromatography. J Dairy Sci. 1966 Jun;49(6):624–627. doi: 10.3168/jds.S0022-0302(66)87920-1. [DOI] [PubMed] [Google Scholar]
- Bricknell K. S., Finegold S. M. A simple, rapid method to process and assay fatty acids and alcohols by gas chromatography. Anal Biochem. 1973 Jan;51(1):23–31. doi: 10.1016/0003-2697(73)90449-1. [DOI] [PubMed] [Google Scholar]
- Drasar B. S., Goddard P., Heaton S., Peach S., West B. Clostridia isolated from faeces. J Med Microbiol. 1976 Feb;9(1):63–71. doi: 10.1099/00222615-9-1-63. [DOI] [PubMed] [Google Scholar]
- Hauser K. J., Zabransky R. J. Modification of the gas-liquid chromatography procedure and evaluation of a new column packing material for the identification of anaerobic bacteria. J Clin Microbiol. 1975 Jul;2(1):1–7. doi: 10.1128/jcm.2.1.1-7.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Norrman J. Production of volatile organic compounds by the yeast fungus Dipodascus aggregatus. Arch Mikrobiol. 1969 Oct;68(2):133–149. doi: 10.1007/BF00413873. [DOI] [PubMed] [Google Scholar]
- Yoshioka, Kitamura M., Tamura Z. Rapid gas chromatographic analysis of microbial volatile metabolites. Jpn J Microbiol. 1969 Mar;13(1):87–93. doi: 10.1111/j.1348-0421.1969.tb00438.x. [DOI] [PubMed] [Google Scholar]