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. 1978 May;7(5):463–466. doi: 10.1128/jcm.7.5.463-466.1978

Bacitracin and coagglutination for grouping of beta-hemolytic streptococci.

R A Stoner
PMCID: PMC275020  PMID: 350895

Abstract

Bacitracin may be used for presumptive differentiation of group A from other beta-hemolytic streptococci. Whatever criteria are used for the test, a small number of erroneous results will be obtained. With the 1,161 strains studied in this laboratory, using a zone of 10 mm or greater as indicative of group A was better than using Maxted's original criterion of any zone of inhibition for group A. Co-agglutination is a preferable alternative to bacitracin testing, providing a grouping result as quickly as the bacitracin test but with the advantage of giving a definite grouping result. With the 247 strains studied so far, coagglutination results have correlated exactly with results of conventional grouping by a precipitin method.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Arvilommi H. Grouping of beta-haemolytic streptococci by using coagglutination, precipitation or bacitracin sensitivity. Acta Pathol Microbiol Scand B. 1976 Apr;84(2):79–84. doi: 10.1111/j.1699-0463.1976.tb01906.x. [DOI] [PubMed] [Google Scholar]
  2. Chitwood L. A., Jennings M. B., Riley H. D., Jr Time, cost, and efficacy study of identifying group A streptococci with commercially available reagents. Appl Microbiol. 1969 Aug;18(2):193–197. doi: 10.1128/am.18.2.193-197.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Christensen P., Kahlmeter G., Jonsson S., Kronvall G. New method for the serological grouping of Streptococci with specific antibodies adsorbed to protein A-containing staphylococci. Infect Immun. 1973 Jun;7(6):881–885. doi: 10.1128/iai.7.6.881-885.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Coleman D. J., McGhie D., Tebbutt G. M. Further studies on the reliability of the bacitracin inhibition test for the presumptive identification of Lancefield group A streptococci. J Clin Pathol. 1977 May;30(5):421–426. doi: 10.1136/jcp.30.5.421. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. ESTELA L. A., SHUEY H. E. COMPARISON OF FLUORESCENT ANTIBODY, PRECIPITIN, AND BACITRACIN DISK METHODS IN THE IDENTIFICATION OF GROUP A STREPTOCOCCI. Am J Clin Pathol. 1963 Dec;40:591–597. doi: 10.1093/ajcp/40.6.591. [DOI] [PubMed] [Google Scholar]
  6. Ederer G. M., Herrmann M. M., Bruce R., Matsen J. M., Chapman S. S. Rapid extraction method with pronase B for grouping beta-hemolytic streptococci. Appl Microbiol. 1972 Feb;23(2):285–288. doi: 10.1128/am.23.2.285-288.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Facklam R. R., Padula J. F., Thacker L. G., Wortham E. C., Sconyers B. J. Presumptive identification of group A, B, and D streptococci. Appl Microbiol. 1974 Jan;27(1):107–113. doi: 10.1128/am.27.1.107-113.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Hahn G., Nyberg I. Identification of streptococcal groups A,B,C, and G by slide co-agglutination of antibody-sensitized protein A-containing staphylococci. J Clin Microbiol. 1976 Jul;4(1):99–101. doi: 10.1128/jcm.4.1.99-101.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. LEVINSON M. L., FRANK P. F. Differentiation of group A from other beta hemolytic streptococci with bacitracin. J Bacteriol. 1955 Mar;69(3):284–287. doi: 10.1128/jb.69.3.284-287.1955. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. MAXTED W. R. The use of bacitracin for identifying group A haemolytic streptococci. J Clin Pathol. 1953 Aug;6(3):224–226. doi: 10.1136/jcp.6.3.224. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Murray P. R., Wold A. D., Hall M. M., Washington J. A., 2nd Bacitracin differentiation for presumptive identification of group A beta-hemolytic streptococci: comparison of primary and purified plate testing. J Pediatr. 1976 Oct;89(4):576–579. doi: 10.1016/s0022-3476(76)80389-7. [DOI] [PubMed] [Google Scholar]
  12. Pollock H. M., Dahlgren B. J. Distribution of streptococcal groups in clinical specimens with evaluation of bacitracin screening. Appl Microbiol. 1974 Jan;27(1):141–143. doi: 10.1128/am.27.1.141-143.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. SCHAUB I. G., MAZEIKA I., LEE R., DUNN M. T., LACHAINE R-A, PRICE W. H. Ecologic studies of rheumatic fever and rheumatic heart disease. I. Procedure for isolating beta hemolytic streptococci. Am J Hyg. 1958 Jan;67(1):46–56. doi: 10.1093/oxfordjournals.aje.a119918. [DOI] [PubMed] [Google Scholar]

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