Abstract
The API 20E system for Enterobacteriaceae, recently broadened to include identification of nonfermentative gram-negative bacteria, was evaluated and compared with the conventional method for complete identification of 221 nonfermenters, which were well distributed into 48 species or biotypes and included organisms not listed in the API 20E data base. The results of 16 tests common to both systems were in close agreement. The API 20E system correctly identified 71 (43%) of the 165 organisms included in the API 20E data base. However, almost 90% of Acinetobacter calcoaceticus, three species of Pseudomonas, and Bordetella bronchiseptica were correctly identified to species.
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Selected References
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- Brooks K. A., Jens M., Sodeman T. M. A clinical evaluation of the API microtube system for identification of Enterobacteriaceae. Am J Med Technol. 1974 Feb;40(2):55–61. [PubMed] [Google Scholar]
- Dowda H. Evaluation of two rapid methods for identification of commonly encountered nonfermenting or oxidase-positive, Gram-negative rods. J Clin Microbiol. 1977 Dec;6(6):605–609. doi: 10.1128/jcm.6.6.605-609.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Nord C. E., Lindberg A. A., Dahlbäck A. Evaluation of five test-kits-API, AuxoTab, Enterotube, PathoTec and R-B-for identification of Enterobacteriaceae. Med Microbiol Immunol. 1974 Mar 22;159(3):211–220. doi: 10.1007/BF02121337. [DOI] [PubMed] [Google Scholar]
- Nord C. E., Wretlind B., Dahlbäck A. Evaluation of two test-kits--API and Oxi Ferm tube--for identification of oxidative-fermentative Gram-negative rods. Med Microbiol Immunol. 1977 Jul 18;163(2):93–97. doi: 10.1007/BF02121824. [DOI] [PubMed] [Google Scholar]
- Robertson E. A., MacLowry J. D. Mathematical analysis of the API enteric 20 profile register using a computer diagnostic model. Appl Microbiol. 1974 Oct;28(4):691–695. doi: 10.1128/am.28.4.691-695.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Shayegani M., Lee A. M., McLynn D. M. Evaluation of the Oxi/Ferm tube system for identification of nonfermentative Gram-negative bacilli. J Clin Microbiol. 1978 Jun;7(6):533–538. doi: 10.1128/jcm.7.6.533-538.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Shayegani M., Lee A. M., Parsons L. M. A scheme for identification of nonfermentative gram-negative bacteria. Health Lab Sci. 1977 Apr;14(2):83–94. [PubMed] [Google Scholar]
- Smith P. B., Tomfohrde K. M., Rhoden D. L., Balows A. API system: a multitube micromethod for identification of Enterobacteriaceae. Appl Microbiol. 1972 Sep;24(3):449–452. doi: 10.1128/am.24.3.449-452.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Washington J. A., 2nd, Yu P. K., Martin W. J. Evaluation of accuracy of multitest micromethod system for identification of Enterobacteriaceae. Appl Microbiol. 1971 Sep;22(3):267–269. doi: 10.1128/am.22.3.267-269.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]