Skip to main content
Journal of Clinical Microbiology logoLink to Journal of Clinical Microbiology
. 1975 Dec;2(6):482–485. doi: 10.1128/jcm.2.6.482-485.1975

Detection of Aspergillus fumigatus serum precipitins by counterimmunoelectrophoresis.

T H Dee
PMCID: PMC275208  PMID: 812884

Abstract

Detection of serum precipitins to Aspergillus fumigatus by counterimmunoelectrophoresis is compared with the immunodiffusion technique. Eight of nine (89%) sera from patients with proven A. fumigatus infection were positive by both methods. No serum from subjects with other systemic mycoses, bacterial infections, or healthy controls had detectable precipitins. The highest serum precipitin titers were found in sera of patients with the mycetomal and invasive forms of the disease. Detection of A. fumigatus serum precipitins by counterimmunoelectrophoresis compares favourably with immunodiffusion and has the advantage of significantly reducing the time required for results.

Full text

PDF
485

Images in this article

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Alter H. J., Holland P. V., Purcell R. H. Counterelectrophoresis for detection of hepatitis--associated antigen: methodology and comparison with gel diffusion and complement fixation. J Lab Clin Med. 1971 Jun;77(6):1000–1010. [PubMed] [Google Scholar]
  2. BROENNESTAM R., HALLBERG T. PRECIPITINS AGAINST AN ANTIGEN EXTRACT OF ASPERGILLUS FUMIGATUS IN PATIENTS WITH ASPERGILLOSIS OR OTHER PULMONARY DISEASES. Acta Med Scand. 1965 Apr;177:385–392. [PubMed] [Google Scholar]
  3. Berlin B. S., Pirojboot N. A rapid method for demonstration of precipitating antibody against influenza virus by counterimmunoelectrophoresis. J Infect Dis. 1972 Sep;126(3):345–347. doi: 10.1093/infdis/126.3.345. [DOI] [PubMed] [Google Scholar]
  4. Biguet J., Fruit J., Vernes A., Capron A. La réaction de fixation du complément et l'immunoélectrophorèse appliquées au diagnostic immunologique de l'aspergillose pulmonaire. Rev Immunol (Paris) 1970 Jul-Sep;34(4):193–203. [PubMed] [Google Scholar]
  5. CAMPBELL M. J., CLAYTON Y. M. BRONCHOPULMONARY ASPERGILLOSIS. A CORRELATION OF THE CLINICAL AND LABORATORY FINDINGS IN 272 PATIENTS INVESTIGATED FOR BRONCHOPULMONARY ASPERGILLOSIS. Am Rev Respir Dis. 1964 Feb;89:186–196. doi: 10.1164/arrd.1964.89.2.186. [DOI] [PubMed] [Google Scholar]
  6. Coleman R. M., Kaufman L. Use of the immunodiffusion test in the serodiagnosis of aspergillosis. Appl Microbiol. 1972 Feb;23(2):301–308. doi: 10.1128/am.23.2.301-308.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Coonrod J. D., Rytel M. W. Detection of type-specific pneumococcal antigens by counterimmunoelectrophoresis. I. Methodology and immunologic properties of pneumococcal antigens. J Lab Clin Med. 1973 May;81(5):770–777. [PubMed] [Google Scholar]
  8. Coonrod J. D., Rytel M. W. Determination of aetiology of bacterial meningitis by counter-immunoelectrophoresis. Lancet. 1972 May 27;1(7761):1154–1157. doi: 10.1016/s0140-6736(72)91376-1. [DOI] [PubMed] [Google Scholar]
  9. Dee T. H., Rytel M. W. Clinical application of counterimmunoelectrophoresis in detection of Candida serum precipitins. J Lab Clin Med. 1975 Jan;85(1):161–166. [PubMed] [Google Scholar]
  10. Dorff G. J., Coonrod J. D., Rytel M. W. Detection by immunoelectrophoresis of antigen in sera of patients with pneumococcal bacteraemia. Lancet. 1971 Mar 20;1(7699):578–579. doi: 10.1016/s0140-6736(71)91169-x. [DOI] [PubMed] [Google Scholar]
  11. Flaherty D. K., Barboriak J., Emanuel D., Fink J., Marx J., Moore V., Reed C. E., Roberts R. Multilaboratory comparison of three immunodiffusion methods used for the detection of precipitating antibodies in hypersensitivity pneumonitis. J Lab Clin Med. 1974 Aug;84(2):298–306. [PubMed] [Google Scholar]
  12. Gocke D. J., Howe C. Rapid detection of Australia antigen by counterimmunoelectrophoresis. J Immunol. 1970 Apr;104(4):1031–1034. [PubMed] [Google Scholar]
  13. Hipp S. S., Berns D. S., Tompkins V., Buckley H. R. Latex slide agglutination test for Aspergillus antibodies. Sabouraudia. 1970 Nov;8(3):237–241. doi: 10.1080/00362177085190931. [DOI] [PubMed] [Google Scholar]
  14. LONGBOTTOM J. L., PEPYS J. PULMONARY ASPERGILLOSIS: DIAGNOSTIC AND IMMUNOLOGICAL SIGNIFICANCE OF ANTIGENS AND C-SUBSTANCE IN ASPERGILLUS FUMIGATUS. J Pathol Bacteriol. 1964 Jul;88:141–151. doi: 10.1002/path.1700880119. [DOI] [PubMed] [Google Scholar]
  15. Nagel J. G., Tuazon C. U., Cardella T. A., Sheagren J. N. Teichoic acid serologic diagnosis of staphylococcal endocarditis. Use of gel diffusion and counterimmunoelectrophoretic methods. Ann Intern Med. 1975 Jan;82(1):13–17. doi: 10.7326/0003-4819-82-1-13. [DOI] [PubMed] [Google Scholar]
  16. PEPYS J., RIDDELL R. W., CITRON K. M., CLAYTON Y. M., SHORT E. I. Clinical and immunologic significance of Aspergillus fumigatus in the sputum. Am Rev Respir Dis. 1959 Aug;80:167–180. doi: 10.1164/arrd.1959.80.2.167. [DOI] [PubMed] [Google Scholar]
  17. Warnock D. W. Indirect immunofluorescence test for the detection of Aspergillus fumigatus antibodies. J Clin Pathol. 1974 Nov;27(11):911–912. doi: 10.1136/jcp.27.11.911. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Journal of Clinical Microbiology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES