Skip to main content
PMC home page
Journal of Clinical Microbiology logoLink to Journal of Clinical Microbiology
. 1978 Sep;8(3):283–287. doi: 10.1128/jcm.8.3.283-287.1978

Measurement of rotavirus antibody by an enzyme-linked immunosorbent assay blocking assay.

R H Yolken, R G Wyatt, B A Barbour, H W Kim, A Z Kapikian, R M Chanock
PMCID: PMC275232  PMID: 212453

Abstract

A new method for the measurement of rotavirus antibody is described, utilizing the system of enzyme-linked immunosorbent assay (ELISA). In this method, serum is incubated with a fixed amount of rotavirus antigen, and the amount of antibody is determined by measuring the amount of unneutralized antigen. Such an assay system proved to be as efficient as the other available rotaviral antibody systems. The ELISA blocking assay also has the advantages of not requiring purified or gnotobiotic antigen and of being able to measure rotaviral antibody in all animal species.

Full text

PDF
285

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Clem T. R., Yolken R. H. Practical colorimeter for direct measurement of microplates in enzyme immunoassay systems. J Clin Microbiol. 1978 Jan;7(1):55–58. doi: 10.1128/jcm.7.1.55-58.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Davidson G. P., Bishop R. F., Townley R. R., Holmes I. H. Importance of a new virus in acute sporadic enteritis in children. Lancet. 1975 Feb 1;1(7901):242–246. doi: 10.1016/s0140-6736(75)91140-x. [DOI] [PubMed] [Google Scholar]
  3. Hamilton J. R., Gall D. G., Kerzner B., Butler D. G., Middleton P. J. Recent developments in viral gastroenteritis. Pediatr Clin North Am. 1975 Nov;22(4):747–755. doi: 10.1016/S0031-3955(16)33205-9. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Kapikian A. Z., Cline W. L., Kim H. W., Kalica A. R., Wyatt R. G., Vankirk D. H., Chanock R. M., James H. D., Jr, Vaughn A. L. Antigenic relationships among five reovirus-like (RVL) agents by complement fixation (CF) and development of new substitute CF antigens for the human RVL agent of infantile gastroenteritis. Proc Soc Exp Biol Med. 1976 Sep;152(4):535–539. doi: 10.3181/00379727-152-39434. [DOI] [PubMed] [Google Scholar]
  5. Kapikian A. Z., Cline W. L., Mebus C. A., Wyatt R. G., Kalica A. R., James H. D., Jr, VanKirk D., Chanock R. M. New complement-fixation test for the human reovirus-like agent of infantile gastroenteritis. Nebraska calf diarrhea virus used as antigen. Lancet. 1975 May 10;1(7915):1056–1061. doi: 10.1016/s0140-6736(75)91827-9. [DOI] [PubMed] [Google Scholar]
  6. Kapikian A. Z., Kim H. W., Wyatt R. G., Cline W. L., Arrobio J. O., Brandt C. D., Rodriguez W. J., Sack D. A., Chanock R. M., Parrott R. H. Human reovirus-like agent as the major pathogen associated with "winter" gastroenteritis in hospitalized infants and young children. N Engl J Med. 1976 Apr 29;294(18):965–972. doi: 10.1056/NEJM197604292941801. [DOI] [PubMed] [Google Scholar]
  7. Kapikian A. Z., Kim H. W., Wyatt R. G., Rodriguez W. J., Ross S., Cline W. L., Parrott R. H., Chanock R. M. Reoviruslike agent in stools: association with infantile diarrhea and development of serologic tests. Science. 1974 Sep 20;185(4156):1049–1053. doi: 10.1126/science.185.4156.1049. [DOI] [PubMed] [Google Scholar]
  8. Kim H. W., Brandt C. D., Kapikian A. Z., Wyatt R. G., Arrobio J. O., Rodriguez W. J., Chanock R. M., Parrott R. H. Human reovirus-like agent infection. Occurrence in adult contacts of pediatric patients with gastroenteritis. JAMA. 1977 Aug 1;238(5):404–407. doi: 10.1001/jama.238.5.404. [DOI] [PubMed] [Google Scholar]
  9. Mebus C. A., Wyatt R. G., Sharpee R. L., Sereno M. M., Kalica A. R., Kapikian A. Z., Twiehaus M. J. Diarrhea in gnotobiotic calves caused by the reovirus-like agent of human infantile gastroenteritis. Infect Immun. 1976 Aug;14(2):471–474. doi: 10.1128/iai.14.2.471-474.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Sibert J. R., Craft A. W., Jackson R. H. Child-resistant packaging and accidental child poisoning. Lancet. 1977 Aug 6;2(8032):289–290. doi: 10.1016/s0140-6736(77)90966-7. [DOI] [PubMed] [Google Scholar]
  11. Torres-Medina A., Wyatt R. G., Mebus C. A., Underdahl N. R., Kapikian A. Z. Diarrhea caused in gnotobiotic piglets by the reovirus-like agent of human infantile gastroenteritis. J Infect Dis. 1976 Jan;133(1):22–27. doi: 10.1093/infdis/133.1.22. [DOI] [PubMed] [Google Scholar]
  12. Voller A., Bidwell D. E., Bartlett A. Enzyme immunoassays in diagnostic medicine. Theory and practice. Bull World Health Organ. 1976;53(1):55–65. [PMC free article] [PubMed] [Google Scholar]
  13. Wyatt R. G., Gill V. W., Sereno M. M., Kalica A. R., VanKirk D. H., Chanock R. M., Kapikian A. Z. Letter: Probable in-vitro cultivation of human reovirus-like agent of infantile diarroea. Lancet. 1976 Jan 10;1(7950):98–99. doi: 10.1016/s0140-6736(76)90202-6. [DOI] [PubMed] [Google Scholar]
  14. Yolken R. H., Barbour B., Wyatt R. G., Kalica A. R., Kapikian A. Z., Chanock R. M. Enzyme-linked immunosorbent assay for identification of rotaviruses from different animal species. Science. 1978 Jul 21;201(4352):259–262. doi: 10.1126/science.208150. [DOI] [PubMed] [Google Scholar]
  15. Yolken R. H., Kim H. W., Clem T., Wyatt R. G., Kalica A. R., Chanock R. M., Kapikian A. Z. Enzyme-linked immunosorbent assay (ELISA) for detection of human reovirus-like agent of infantile gastroenteritis. Lancet. 1977 Aug 6;2(8032):263–267. doi: 10.1016/s0140-6736(77)90951-5. [DOI] [PubMed] [Google Scholar]
  16. Yolken R. H., Wyatt R. G., Kim H. W., Kapikian A. Z., Chanock R. M. Immunological response to infection with human reovirus-like agent: measurement of anti-human reovirus-like agent immunoglobulin G and M levels by the method of enzyme-linked immunosorbent assay. Infect Immun. 1978 Feb;19(2):540–546. doi: 10.1128/iai.19.2.540-546.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Zissis G., Lambert J. P. Different serotypes of human rotaviruses. Lancet. 1978 Jan 7;1(8054):38–39. doi: 10.1016/s0140-6736(78)90380-x. [DOI] [PubMed] [Google Scholar]

Articles from Journal of Clinical Microbiology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES