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. 1978 Dec;8(6):748–755. doi: 10.1128/jcm.8.6.748-755.1978

Phagocytic resistance of Escherichia coli K-1 isolates and relationship to virulence.

R Weinstein, L S Young
PMCID: PMC275336  PMID: 370149

Abstract

Blood culture isolates from 133 episodes of Escherichia coli bacteremia were typed for K-1 capsular antigen by immunodiffusion, utilizing equine antiserum raised against meningococcal group B polysaccharide. Twenty-six percent (34 of 133) of these isolates were positive for K-1 antigen. These 133 strains, 34 K-1 and 99 non-K-1, were tested for susceptibility to phagocytosis. K-1 strains were found to be more resistant to clearance (27%) than non-K-1 strains (71%) when tested in an in vitro opsonophagocytic/killing assay containing normal human granulocytes and plasma. Additional studies demonstrated that resistance was due to decreased phagocytosis rather than diminished intraleukocytic killing. K-1 strains obtained from stool showed a similar degree of resistance to phagocytosis when compared with K-1 blood isolates. A comparison of clinical data on non-neonatal patients with E. coli K-1 and non-K-1 bacteremia showed no significant differences in mortality for these two groups. The incidence of shock for patients bacteremic with K-1 strains (74%) was significantly greater than that for patients bacteremic with non-K-1 strains (33%). These differences are attributed to the increased resistance to phagocytosis observed for K-1 versus non-K-1 E. coli isolates.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Fine D. P., Marney S. R., Jr, Colley D. G., Sergent J. S., Des Prez R. M. C3 shunt activation in human serum chelated with EGTA. J Immunol. 1972 Oct;109(4):807–809. [PubMed] [Google Scholar]
  2. HIRSCH J. G., STRAUSS B. STUDIES ON HEAT-LABILE OPSONIN IN RABBIT SERUM. J Immunol. 1964 Jan;92:145–154. [PubMed] [Google Scholar]
  3. Howard C. J., Glynn A. A. The virulence for mice of strains of Escherichia coli related to the effects of K antigens on their resistance to phagocytosis and killing by complement. Immunology. 1971 May;20(5):767–777. [PMC free article] [PubMed] [Google Scholar]
  4. Kaijser B., Ahlstedt S. Protective capacity of antibodies against Escherichia coli and K antigens. Infect Immun. 1977 Aug;17(2):286–289. doi: 10.1128/iai.17.2.286-289.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Kaijser B. Immunology of Escherichia coli: K antigen and its relation to urinary-tract infection. J Infect Dis. 1973 Jun;127(6):670–677. doi: 10.1093/infdis/127.6.670. [DOI] [PubMed] [Google Scholar]
  6. Kaijser B., Jodal U., Hanson L. A. Studies on antibody response and tolerance to E, coli K antigens in immunized rabbits and in children with urinary tract infection. Int Arch Allergy Appl Immunol. 1973;44(2):260–273. doi: 10.1159/000230935. [DOI] [PubMed] [Google Scholar]
  7. Lidin-Janson G., Hanson L. A., Kaijser B., Lincoln K., Lindberg U., Olling S., Wedel H. Comparison of Escherichia coli from bacteriuric patients with those from feces of healthy schoolchildren. J Infect Dis. 1977 Sep;136(3):346–353. doi: 10.1093/infdis/136.3.346. [DOI] [PubMed] [Google Scholar]
  8. McCabe W. R., Carling P. C., Bruins S., Greely A. The relation of K-antigen to virulence of Escherichia coli. J Infect Dis. 1975 Jan;131(1):6–10. doi: 10.1093/infdis/131.1.6. [DOI] [PubMed] [Google Scholar]
  9. McCabe W. R., Kreger B. E., Johns M. Type-specific and cross-reactive antibodies in gram-negative bacteremia. N Engl J Med. 1972 Aug 10;287(6):261–267. doi: 10.1056/NEJM197208102870601. [DOI] [PubMed] [Google Scholar]
  10. McCracken G. H., Jr, Sarff L. D., Glode M. P., Mize S. G., Schiffer M. S., Robbins J. B., Gotschlich E. C., Orskov I., Orskov F. Relation between Escherichia coli K1 capsular polysaccharide antigen and clinical outcome in neonatal meningitis. Lancet. 1974 Aug 3;2(7875):246–250. doi: 10.1016/s0140-6736(74)91413-5. [DOI] [PubMed] [Google Scholar]
  11. Robbins J. B., McCracken G. H., Jr, Gotschlich E. C., Orskov F., Orskov I., Hanson L. A. Escherichia coli K1 capsular polysaccharide associated with neonatal meningitis. N Engl J Med. 1974 May 30;290(22):1216–1220. doi: 10.1056/NEJM197405302902202. [DOI] [PubMed] [Google Scholar]
  12. Weinstein R. J., Young L. S. Neutrophil function in gram-negative rod bacteremia. The interaction between phagocytic cells, infecting organisms, and humoral factors. J Clin Invest. 1976 Jul;58(1):190–199. doi: 10.1172/JCI108449. [DOI] [PMC free article] [PubMed] [Google Scholar]

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