Abstract
Kessel, R. W. I. (Rutgers, The State University, New Brunswick, N.J.), Henry H. Freedman, and Werner Braun. Relation of polysaccharide content to some biological properties of endotoxins from mutants of Salmonella typhimurium. J. Bacteriol. 92:592–596. 1966.—Endotoxins were extracted by the phenol-water procedure from a variety of Salmonella typhimurium mutants with known differences in the composition of their cell wall polysaccharides. The lethality of these preparations for mice proved to be correlated with the complexity of the polysaccharide: endotoxin from the smooth parent strain and from rough strains with several sugars attached to the heptose-phosphate backbone were of high toxicity, whereas endotoxin from a mutant possessing only glucose attached to the heptose-phosphate backbone was less toxic, and endotoxin from a mutant possessing the backbone only was least toxic. All of these mutants yielded endotoxins that were equally capable of protecting mice against subsequent challenge with Pseudomonas aeruginosa. Material obtained from a heptoseless mutant by the phenol-water method proved to be neither toxic nor protective. The apparent dissociation of biological properties that can be achieved with the aid of endotoxin preparations from certain mutants is discussed in terms of possible mechanisms.
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Selected References
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- DISCHE Z. New color reactions for determination of sugars in polysaccharides. Methods Biochem Anal. 1955;2:313–358. doi: 10.1002/9780470110188.ch11. [DOI] [PubMed] [Google Scholar]
- Kessel R. W., Braun W. Cytotoxicity of endotoxin in vitro. Effects on macrophages from normal guinea pigs. Aust J Exp Biol Med Sci. 1965 Aug;43(4):511–522. doi: 10.1038/icb.1965.36. [DOI] [PubMed] [Google Scholar]
- Kessel R. W., Braun W., Plescia O. J. Endotoxin cytotoxicity: role of cell-associated antibody. Proc Soc Exp Biol Med. 1966 Feb;121(2):449–452. doi: 10.3181/00379727-121-30801. [DOI] [PubMed] [Google Scholar]
- Lüderitz O., Staub A. M., Westphal O. Immunochemistry of O and R antigens of Salmonella and related Enterobacteriaceae. Bacteriol Rev. 1966 Mar;30(1):192–255. doi: 10.1128/br.30.1.192-255.1966. [DOI] [PMC free article] [PubMed] [Google Scholar]
- NAKANO M. Mutants of Salmonella with unusually low toxicity for mice. Nature. 1962 Dec 15;196:1118–1119. doi: 10.1038/1961118a0. [DOI] [PubMed] [Google Scholar]
- NIKAIDO H., MIKAIDO K., SUBBAIAH T. V., STOCKER B. A. ROUGH MUTANTS OF SALMONELLA TYPHIMURIUM. III. ENZYMATIC SYNTHESIS OF NUCLEOTIDE-SUGAR COMPOUNDS. Nature. 1964 Mar 28;201:1301–1302. doi: 10.1038/2011301a0. [DOI] [PubMed] [Google Scholar]
- OSBORN M. J., ROSEN S. M., ROTHFIELD L., ZELEZNICK L. D., HORECKER B. L. LIPOPOLYSACCHARIDE OF THE GRAM-NEGATIVE CELL WALL. Science. 1964 Aug 21;145(3634):783–789. doi: 10.1126/science.145.3634.783. [DOI] [PubMed] [Google Scholar]
- SUBBAIAH T. V., STOCKER B. A. ROUGH MUTANTS OF SALMONELLA TYPHIMURIUM. I. GENETICS. Nature. 1964 Mar 28;201:1298–1299. doi: 10.1038/2011298a0. [DOI] [PubMed] [Google Scholar]
- SULTZER B. M., FREEDMAN H. H. The stimulation of non-specific host resistance to infection by chemically modified endotoxin. J Exp Med. 1962 Dec 1;116:943–956. doi: 10.1084/jem.116.6.943. [DOI] [PMC free article] [PubMed] [Google Scholar]