Skip to main content
PMC home page
Journal of Bacteriology logoLink to Journal of Bacteriology
. 1966 Oct;92(4):899–904. doi: 10.1128/jb.92.4.899-904.1966

Membrane-associated Sulfur Oxidation by the Autotroph Thiobacillus thiooxidans

Frank W Adair 1
PMCID: PMC276351  PMID: 5926757

Abstract

Adair, Frank W. (Rutgers, The State University, New Brunswick, N. J.). Membrane-associated sulfur oxidation by the autotroph Thiobacillus thiooxidans. J. Bacteriol. 92:899–904. 1966.—Washed cell wall-membrane fragments derived from sulfur-grown cells of the strictly autotrophic bacterium, Thiobacillus thiooxidans, oxidized elemental sulfur to sulfate without the addition of cofactors. The oxidation was optimal at pH 7.0 and was increased by the presence of wetting agents. Oxygen uptake was inhibited by cyanide, azide, and thiol-binding agents. Sulfite was also oxidized, and both the sulfur- and sulfite-oxidizing systems were heat-labile. Neither thiosulfate nor tetrathionate was oxidized by soluble or membrane preparations. The fragments fixed C14O2 in the presence of ribose-5-phosphate, Mg++, and adenosine triphosphate. Sulfur oxidation did not provide energy for C14O2 fixation in this system.

Full text

PDF
901

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. COOK T. M. GROWTH OF THIOBACILLUS THIOOXIDANS IN SHAKEN CULTURE. J Bacteriol. 1964 Sep;88:620–623. doi: 10.1128/jb.88.3.620-623.1964. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. COOK T. M., UMBREIT W. W. The occurrence of cytochrome and coenzyme Q in Thiobacillus thiooxidans. Biochemistry. 1963 Jan-Feb;2:194–196. doi: 10.1021/bi00901a036. [DOI] [PubMed] [Google Scholar]
  3. JONES G. E., BENSON A. A. PHOSPHATIDYL GLYCEROL IN THIOBACILLUS THIOOXIDANS. J Bacteriol. 1965 Jan;89:260–261. doi: 10.1128/jb.89.1.260-261.1965. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. JONES G. E., STARKEY R. L. Surface-active substances produced by Thiobacillus thiooxidans. J Bacteriol. 1961 Nov;82:788–789. doi: 10.1128/jb.82.5.788-789.1961. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. LONDON J., RITTENBERG S. C. PATH OF SULFUR IN SULFIDE AND THIOSULFATE OXIDATION BY THIOBACILLI. Proc Natl Acad Sci U S A. 1964 Nov;52:1183–1190. doi: 10.1073/pnas.52.5.1183. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. London J. Cytochrome in Thiobacillus thiooxidans. Science. 1963 Apr 26;140(3565):409–410. doi: 10.1126/science.140.3565.409. [DOI] [PubMed] [Google Scholar]
  7. PECK H. D., Jr, FISHER E., Jr The oxidation of thiosulfate and phosphorylation in extracts of Thiobacillus thioparus. J Biol Chem. 1962 Jan;237:190–197. [PubMed] [Google Scholar]
  8. SCHAEFFER W. I., HOLBERT P. E., UMBREIT W. W. Attachment of Thiobacillus thiooxidans to sulfur crystals. J Bacteriol. 1963 Jan;85:137–140. doi: 10.1128/jb.85.1.137-140.1963. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. SCHAEFFER W. I., UMBREIT W. W. Phosphotidylinositol as a wetting agent in sulfur oxidation by Thiobacillus thiooxidans. J Bacteriol. 1963 Feb;85:492–493. doi: 10.1128/jb.85.2.492-493.1963. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. SUZUKI I., WERKMAN C. H. Chemoautotrophic carbon dioxide fixation by extracts of Thiobacillus thiooxidans. II. Formation of phosphoglyceric acid. Arch Biochem Biophys. 1958 Sep;77(1):112–123. doi: 10.1016/0003-9861(58)90047-x. [DOI] [PubMed] [Google Scholar]
  11. Suzuki I. Incorporation of atmospheric oxygen-18 into thiosulfate by the sulfur-oxidizing enzyme of Thiobacillus thiooxidans. Biochim Biophys Acta. 1965 Oct 25;110(1):97–101. doi: 10.1016/s0926-6593(65)80098-4. [DOI] [PubMed] [Google Scholar]
  12. Suzuki I. Oxidation of elemental sulfur by an enzyme system of Thiobacillus thiooxidans. Biochim Biophys Acta. 1965 Jul 8;104(2):359–371. doi: 10.1016/0304-4165(65)90341-7. [DOI] [PubMed] [Google Scholar]
  13. Suzuki I., Werkman C. H. GLUTATHIONE AND SULFUR OXIDATION BY THIOBACILLUS THIOOXIDANS. Proc Natl Acad Sci U S A. 1959 Feb;45(2):239–244. doi: 10.1073/pnas.45.2.239. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. TRUDINGER P. A. EFFECT OF THIOL-BINDING REAGENTS ON THE METABOLISM OF THIOSULFATE AND TETRATHIONATE BY THIOBACILLUS NEAPOLITANUS. J Bacteriol. 1965 Mar;89:617–625. doi: 10.1128/jb.89.3.617-625.1965. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Journal of Bacteriology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES