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. 1966 Oct;92(4):1099–1110. doi: 10.1128/jb.92.4.1099-1110.1966

Ribonuclease Sensitivity of Escherichia coli Ribosomes

Melvin Santer 1, Josephine R Smith 1
PMCID: PMC276384  PMID: 5332866

Abstract

Santer, Melvin (Haverford College, Haverford, Pa.), and Josephine R. Smith. Ribonuclease sensitivity of Escherichia coli ribosomes. J. Bacteriol. 92:1099–1110. 1966.—The ribonucleic acid (RNA) contained in 70S ribosomes and in 50S and 30S subunits was hydrolyzed by pancreatic ribonuclease. A 7% amount of the RNA was removed from the 70S particle; at 10−4m magnesium concentration, a maximum of 24 and 30% of the RNA in the 50S and the 30S fractions, respectively, was removed by ribonuclease. At the two lower magnesium ion concentrations, 50S ribosomes did not lose any protein, whereas 30S ribosomes lost protein as a result of ribonuclease treatment. A number of proteins were removed from the 30S particles by ribonuclease, and these proteins were antigenically related to proteins present in 50S ribosomes. The differential effect of ribonuclease on 50S and 30S ribosomes suggested that they have structural dissimilarities.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. CANNON M., KRUG R., GILBERT W. THE BINDING OF S-RNA BY ESCHERICHIA COLI RIBOSOMES. J Mol Biol. 1963 Oct;7:360–378. doi: 10.1016/s0022-2836(63)80030-3. [DOI] [PubMed] [Google Scholar]
  2. DAVIES J., GILBERT W., GORINI L. STREPTOMYCIN, SUPPRESSION, AND THE CODE. Proc Natl Acad Sci U S A. 1964 May;51:883–890. doi: 10.1073/pnas.51.5.883. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. ENNIS H. L., LUBIN M. PRE-RIBOSOMAL PARTICLES FORMED IN POTASSIUM-DEPLETED CELLS. STUDIES ON DEGRADATION AND STABILIZATION. Biochim Biophys Acta. 1965 Apr 19;95:605–623. doi: 10.1016/0005-2787(65)90515-0. [DOI] [PubMed] [Google Scholar]
  4. GILBERT W. Polypeptide synthesis in Escherichia coli. II. The polypeptide chain and S-RNA. J Mol Biol. 1963 May;6:389–403. doi: 10.1016/s0022-2836(63)80051-0. [DOI] [PubMed] [Google Scholar]
  5. KIRBY K. S. A new method for the isolation of ribonucleic acids from mammalian tissues. Biochem J. 1956 Nov;64(3):405–408. doi: 10.1042/bj0640405. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Kaji H., Suzuka I., Kaji A. Binding of specific soluble ribonucleic acid to ribosomes. Binding of soluble ribonucleic acid to the template-30 S subunits complex. J Biol Chem. 1966 Mar 25;241(6):1251–1256. [PubMed] [Google Scholar]
  7. LEBOY P. S., COX E. C., FLAKS J. G. THE CHROMOSOMAL SITE SPECIFYING A RIBOSOMAL PROTEIN IN ESCHERICHIA COLI. Proc Natl Acad Sci U S A. 1964 Dec;52:1367–1374. doi: 10.1073/pnas.52.6.1367. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  9. McPhie P., Hounsell J., Gratzer W. B. The specific cleavage of yeast ribosomal ribonucleic acid with nucleases. Biochemistry. 1966 Mar;5(3):988–993. doi: 10.1021/bi00867a026. [DOI] [PubMed] [Google Scholar]
  10. Neu H. C., Heppel L. A. The release of ribonuclease into the medium when E. coli cells are converted to spheroplasts. Biochem Biophys Res Commun. 1964;14:109–112. doi: 10.1016/0006-291x(64)90238-4. [DOI] [PubMed] [Google Scholar]
  11. Old D., Gorini L. Amino acid changes provoked by streptomycin in a polypeptide synthesized in vitro. Science. 1965 Dec 3;150(3701):1290–1292. doi: 10.1126/science.150.3701.1290. [DOI] [PubMed] [Google Scholar]
  12. Osawa S. Biosynthesis of ribosomes in bacterial cells. Prog Nucleic Acid Res Mol Biol. 1965;4:161–188. doi: 10.1016/s0079-6603(08)60787-4. [DOI] [PubMed] [Google Scholar]
  13. SANTER M. RIBOSOMAL RNA ON THE SURFACE OF RIBOSOMES. Science. 1963 Sep 13;141(3585):1049–1050. doi: 10.1126/science.141.3585.1049. [DOI] [PubMed] [Google Scholar]
  14. SPENCER M., POOLE J. ON THE ORIGIN OF CRYSTALLIZABLE RNA FROM YEAST. J Mol Biol. 1965 Feb;11:314–326. doi: 10.1016/s0022-2836(65)80060-2. [DOI] [PubMed] [Google Scholar]
  15. SYPHERD P. S. ACCUMULATION OF RIBONUCLEOPROTEIN PARTICLES IN A RELAXED MUTANT OF ESCHERICHIA COLI. J Bacteriol. 1965 Aug;90:403–410. doi: 10.1128/jb.90.2.403-410.1965. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. WATSON J. D. Involvement of RNA in the synthesis of proteins. Science. 1963 Apr 5;140(3562):17–26. doi: 10.1126/science.140.3562.17. [DOI] [PubMed] [Google Scholar]

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