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. 1966 Oct;92(4):1133–1140. doi: 10.1128/jb.92.4.1133-1140.1966

Persistent Infection of a Rat Kidney Cell Line with Rauscher Murine Leukemia Virus

Huu Duc-Nguyen 1, Edith N Rosenblum 1, Robert F Zeigel 1
PMCID: PMC276388  PMID: 5926742

Abstract

Duc-Nguyen, Huu (National Cancer Institute, Bethesda, Md.), Edith N. Rosenblum, and Robert F. Zeigel. Persistent infection of a rat kidney cell line with Rauscher murine leukemia virus. J. Bacteriol. 92:1133–1140. 1966.—The propagation of a murine leukemia virus (Rauscher) in a kidney cell line, derived from a rat with lymphoid leukemia, was studied. A complement-fixing (CF) antigen reacting with Rauscher immune sera was detected at various passage levels, which correlated with the visualization by use of electron microscopy of viral buds and viral particles in different stages of maturation in all passages. Five-month-old monolayers continued to shed virus and to yield high CF antigen titers. The cell-free supernatant fluid from cultures of the 14th passage was shown to be infectious for a normal rat kidney cell line, as evidenced by the appearance of the CF antigen in this line. Interferon production was not demonstrated in infected cultures. The overall data indicated that rat kidney cells could be used to propagate Rauscher virus in a carrier state.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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