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. 1967 Mar;93(3):874–878. doi: 10.1128/jb.93.3.874-878.1967

Yield Coefficients of Thiobacillus neapolitanus in Continuous Culture

Walter P Hempfling a,1, Wolf Vishniac a
PMCID: PMC276530  PMID: 6025430

Abstract

Thiobacillus neapolitanus, when grown in continuous culture with thiosulfate limiting growth, possessed an apparent maximal molar growth yield of 8.0 g (dry weight) per mole of thiosulfate. The substrate requirement for energy of maintenance was the highest yet reported, amounting to 21.8 mmoles of thiosulfate per g per hr. The molar growth yield, corrected for this maintenance energy requirement, was 13.9 g (dry weight) per mole of thiosulfate. It was concluded that substrate-level phosphorylation during sulfite oxidation accounted for about 45% of the adenosine triphosphate (ATP) requirement for CO2 assimilation and maintenance during growth on limiting thiosulfate, that three sites of energy conservation exist in the electron-transport chain terminating in oxygen, and that 7.8 moles of ATP are required to fix and assimilate 1 mole of CO2 into cell material.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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