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. 1967 Jun;93(6):2003–2008. doi: 10.1128/jb.93.6.2003-2008.1967

Purification and Chemical Composition of Reticulate Bodies of the Meningopneumonitis Organisms

Akira Tamura 1, Akira Matsumoto 1, Noboru Higashi 1
PMCID: PMC276720  PMID: 6025310

Abstract

Reticulate bodies of the meningopneumonitis (MP) microorganism were purified from L cells 18 hr after infection by the combination of differential centrifugation in 30% sucrose solution and potassium tartrate density gradient centrifugation. It was ascertained by electron microscopy that purified preparations of reticulate bodies obtained were almost entirely free of host-cell components and of infectious elementary bodies of MP microorganisms. Purified reticulate bodies were easily disrupted by mechanical agitation, and it was observed in shadowed preparation that ribosome-like particles 15 mμ in diameter were scattered from broken reticulate bodies. In shadowed preparations, reticulate bodies were found to range in size from 1.0 to 1.6 μ in diameter, but in cross-section the range was 0.5 to 1.0 μ. In these preparations, the purified reticulate bodies were irregular in shape, round or oval, and were composed of rather homogenous, amorphous, or reticulate material with moderate density. Some particles exhibited a less-dense internal structure, in which a coarse fibrous reticulum was seen. Chemical fractionation of 32P-labeled purified reticulate bodies showed that they contained three times more ribonucleic acid (RNA) than deoxyribonucleic acid, with the RNA being composed primarily of 21S, 16S, and 4S RNA. No infectivity of purified reticulate bodies could be demonstrated.

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Selected References

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  1. ALLEN E. G., BOVARNICK M. R. Association of reduced diphosphopyridine nucleotide cytochrome c reductase activity with meningopneumonitis virus. J Exp Med. 1957 Jun 1;105(6):539–547. doi: 10.1084/jem.105.6.539. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Allen E. G. Use of tetrazolium salts for electron transport studies in meningopneumonitis. I. Reduced nicotinamide adenine dinucleotide system. J Bacteriol. 1965 Dec;90(6):1505–1512. doi: 10.1128/jb.90.6.1505-1512.1965. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. DULBECCO R., VOGT M. Plaque formation and isolation of pure lines with poliomyelitis viruses. J Exp Med. 1954 Feb;99(2):167–182. doi: 10.1084/jem.99.2.167. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. ERLANDSON R. A., ALLEN E. G. THE ULTRASTRUCTURE OF MENINGOPNEUMONITIS. Virology. 1964 Mar;22:410–418. doi: 10.1016/0042-6822(64)90031-5. [DOI] [PubMed] [Google Scholar]
  5. HIGASHI N., TAMURA A. A plaque assay for meningopneumonitis virus in monolayers of strain L cells. Virology. 1960 Dec;12:578–588. doi: 10.1016/0042-6822(60)90180-x. [DOI] [PubMed] [Google Scholar]
  6. HIGASHI N., TAMURA A., IWANAGA M. Developmental cycle and reproductive mechanism of the meningopneumonitis virus in strain L cells. Ann N Y Acad Sci. 1962 Mar 5;98:100–121. doi: 10.1111/j.1749-6632.1962.tb30536.x. [DOI] [PubMed] [Google Scholar]
  7. Higashi N. The mode of reproduction of psittacosis-lymphogranuloma-trachoma (PLT) group viruses. Int Rev Exp Pathol. 1964;3:35–64. [PubMed] [Google Scholar]
  8. JENKIN H. M. Preparation and properties of cell walls of the agent of meningopneumonitis. J Bacteriol. 1960 Nov;80:639–647. doi: 10.1128/jb.80.5.639-647.1960. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. LUFT J. H. Improvements in epoxy resin embedding methods. J Biophys Biochem Cytol. 1961 Feb;9:409–414. doi: 10.1083/jcb.9.2.409. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. MOULDER J. W. Some basic properties of the psittacosis-lymphogranuloma venereum group of agents. Structure and chemical composition of isolated particles. Ann N Y Acad Sci. 1962 Mar 5;98:92–99. doi: 10.1111/j.1749-6632.1962.tb30535.x. [DOI] [PubMed] [Google Scholar]
  11. POLLARD M., STARR T. J., MOORE R. W., TANAMI Y. Cytochemical changes in human amnion cell, infected with psittacosis virus. Nature. 1960 Nov 26;188:770–770. doi: 10.1038/188770a0. [DOI] [PubMed] [Google Scholar]
  12. WEISS E., MYERS W. F., DRESSLER H. R., CHUN-HOON H. GLUCOSE METABOLISM BY AGENTS OF THE PSITTACOSIS-TRACHOMA GROUP. Virology. 1964 Apr;22:551–562. doi: 10.1016/0042-6822(64)90076-5. [DOI] [PubMed] [Google Scholar]

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