Abstract
During experiments on the immunological immobilization of treponemes, several cultured strains were lysed in the guinea pig serum used as a source of complement. In further studies employing Borrelia vincentii, lysis, observed by darkfield, appeared as swelling and some beading of the cells. Complete disruption eventuated. Untreated guinea pig serum was strongly lytic, whereas little or no lysis occurred in bentonite-adsorbed samples. Activity was restored to these samples by adding commercially obtained crystalline egg-white lysozyme. Serum samples which had been heated, absorbed with aggregates of human γ-globulin, or treated with ethylenediaminetetraacetate to remove, or inactivate, complement also lost spirochetolytic activity.
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- EDELMAN G. M., KUNKEL H. G., FRANKLIN E. C. Interaction of the rheumatoid factor with antigen-antibody complexes and aggregated gamma globulin. J Exp Med. 1958 Jul 1;108(1):105–120. doi: 10.1084/jem.108.1.105. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Glynn A. A., Milne C. M. Lysozyme and immune bacteriolysis. Nature. 1965 Sep 18;207(5003):1309–1310. doi: 10.1038/2071309a0. [DOI] [PubMed] [Google Scholar]
- Guest W. J., Nevin T. A., Thomas M. L., Adams J. A. Immobilization of certain cultured treponemes in sera from syphilitic humans. J Bacteriol. 1967 Mar;93(3):1190–1191. doi: 10.1128/jb.93.3.1190-1191.1967. [DOI] [PMC free article] [PubMed] [Google Scholar]
- HOLDEN J. T., VANBALGOOY J. N. EFFECT OF NUTRITIONAL AND PHYSIOLOGICAL FACTORS ON THE REACTION BETWEEN LACTOBACILLUS PLANTARUM AND MURAMIDASE. Biochem Biophys Res Commun. 1965 May 3;19:401–406. doi: 10.1016/0006-291x(65)90136-1. [DOI] [PubMed] [Google Scholar]
- KENT J. F., DE WEERDT J. B. Enhancement by lysozyme of the sensitivity of Treponema pallidum immobilization tests. Br J Vener Dis. 1963 Mar;39:37–40. doi: 10.1136/sti.39.1.37. [DOI] [PMC free article] [PubMed] [Google Scholar]
- LEVINE L., COWAN K. M., OSLER A. G., MAYER M. M. Studies on the role of Ca++ and Mg++ in complement fixation and immune hemolysis. II. The essential role of calcium in complement fixation. J Immunol. 1953 Nov;71(5):367–373. [PubMed] [Google Scholar]
- METZGER M., HARDY P. H., Jr, NELL E. E. Influence of lysozyme upon the treponeme immobilization reaction. Am J Hyg. 1961 Mar;73:236–244. doi: 10.1093/oxfordjournals.aje.a120182. [DOI] [PubMed] [Google Scholar]
- NEVIN T. A., HAMPP E. G. Partially defined medium for the cultivation of Borrelia vincentil. J Bacteriol. 1959 Aug;78:263–266. doi: 10.1128/jb.78.2.263-266.1959. [DOI] [PMC free article] [PubMed] [Google Scholar]
- SMOLELIS A. N., HARTSELL S. E. The determination of lysozyme. J Bacteriol. 1949 Dec;58(6):731–736. doi: 10.1128/jb.58.6.731-736.1949. [DOI] [PMC free article] [PubMed] [Google Scholar]
- WARDLAW A. C. The complement-dependent bacteriolytic activity of normal human serum. I. The effect of pH and ionic strength and the role of lysozyme. J Exp Med. 1962 Jun 1;115:1231–1249. doi: 10.1084/jem.115.6.1231. [DOI] [PMC free article] [PubMed] [Google Scholar]