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. 1967 Nov;94(5):1623–1628. doi: 10.1128/jb.94.5.1623-1628.1967

An Upper Limit on β-Galactosidase Transfer in Bacterial Conjugation1

J L Rosner 1,2,2, Edward A Adelberg 1,2, Michael B Yarmolinsky 1,2
PMCID: PMC276871  PMID: 4862199

Abstract

An upper limit for β-galactosidase transfer between mating F+ and F−Escherichia coli has been determined by a new technique which relies on selective lysis of the donor strain by heat induction of a thermo-inducible strain of λ, accompanied by chymotryptic digestion of the released β-galactosidase. No significant transfer of β-galactosidase during mating between F+ and F− cells has been observed: 0.05 ± 0.05% of the enzyme originally present in the male cells is found in the female cells after 1 hr of mating at 37 C.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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