Skip to main content
PMC home page
Journal of Bacteriology logoLink to Journal of Bacteriology
. 1967 Nov;94(5):1623–1628. doi: 10.1128/jb.94.5.1623-1628.1967

An Upper Limit on β-Galactosidase Transfer in Bacterial Conjugation1

J L Rosner 1,2,2, Edward A Adelberg 1,2, Michael B Yarmolinsky 1,2
PMCID: PMC276871  PMID: 4862199

Abstract

An upper limit for β-galactosidase transfer between mating F+ and F−Escherichia coli has been determined by a new technique which relies on selective lysis of the donor strain by heat induction of a thermo-inducible strain of λ, accompanied by chymotryptic digestion of the released β-galactosidase. No significant transfer of β-galactosidase during mating between F+ and F− cells has been observed: 0.05 ± 0.05% of the enzyme originally present in the male cells is found in the female cells after 1 hr of mating at 37 C.

Full text

PDF

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. ADELBERG E. A., BURNS S. N. Genetic variation in the sex factor of Escherichia coli. J Bacteriol. 1960 Mar;79:321–330. doi: 10.1128/jb.79.3.321-330.1960. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. ANDERSON T. F., WOLLMAN E. L., JACOB F. Sur les processus de conjugaison et de recombinaison chez Escherichia coli. III. Aspects morphologiques en microscopie électronique. Ann Inst Pasteur (Paris) 1957 Oct;93(4):450–455. [PubMed] [Google Scholar]
  3. BRINTON C. C., Jr, GEMSKI P., Jr, CARNAHAN J. A NEW TYPE OF BACTERIAL PILUS GENETICALLY CONTROLLED BY THE FERTILITY FACTOR OF E. COLI K 12 AND ITS ROLE IN CHROMOSOME TRANSFER. Proc Natl Acad Sci U S A. 1964 Sep;52:776–783. doi: 10.1073/pnas.52.3.776. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Doermann A. H. Lysis and Lysis Inhibition with Escherichia coli Bacteriophage. J Bacteriol. 1948 Feb;55(2):257–276. doi: 10.1128/jb.55.2.257-276.1948. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. FISHER K. W. Conjugal transfer of immunity to phage lambda multiplication in Escherichia coli K-12. J Gen Microbiol. 1962 Sep;28:711–719. doi: 10.1099/00221287-28-4-711. [DOI] [PubMed] [Google Scholar]
  6. FISHER K. W. The nature of the endergonic processes in conjugation in Escherichia coli K-12. J Gen Microbiol. 1957 Feb;16(1):136–145. doi: 10.1099/00221287-16-1-136. [DOI] [PubMed] [Google Scholar]
  7. GAREN A., SKAAR P. D. Transfer of phosphorus-containing material associated with mating in Escherichia coli. Biochim Biophys Acta. 1958 Mar;27(3):457–463. doi: 10.1016/0006-3002(58)90372-x. [DOI] [PubMed] [Google Scholar]
  8. Givol D., Craven G. R., Steers E., Jr, Anfinsen C. B. Effect of limited digestion by proteolytic enzymes on Escherichia coli beta-galactosidase. Biochim Biophys Acta. 1966 Jan 11;113(1):120–125. doi: 10.1016/s0926-6593(66)80127-3. [DOI] [PubMed] [Google Scholar]
  9. Gross J. D., Caro L. G. DNA transfer in bacterial conjugation. J Mol Biol. 1966 Apr;16(2):269–284. doi: 10.1016/s0022-2836(66)80172-9. [DOI] [PubMed] [Google Scholar]
  10. HERMAN R. K., FORRO F., Jr AUTORADIOGRAPHIC STUDY OF TRANSFER OF DNA DURING BACTERIAL CONJUGATION. Biophys J. 1964 Sep;4:335–353. doi: 10.1016/s0006-3495(64)86787-4. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. HERSHEY A. D., KAMEN M. D., KENNEDY J. W., GEST H. The mortality of bacteriophage containing assimilated radioactive phosphorus. J Gen Physiol. 1951 Jan;34(3):305–319. doi: 10.1085/jgp.34.3.305. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. JACOB F., MONOD J. Genetic regulatory mechanisms in the synthesis of proteins. J Mol Biol. 1961 Jun;3:318–356. doi: 10.1016/s0022-2836(61)80072-7. [DOI] [PubMed] [Google Scholar]
  13. JACOB F., WOLLMAN E. L. Sur les processus de conjugaison et de recombinaison chez Escherichia coli. I. L'induction par conjugaison ou induction zygotique. Ann Inst Pasteur (Paris) 1956 Oct;91(4):486–510. [PubMed] [Google Scholar]
  14. Jacob F., Ryter A., Cuzin F. On the association between DNA and membrane in bacteria. Proc R Soc Lond B Biol Sci. 1966 Mar 22;164(995):267–278. doi: 10.1098/rspb.1966.0029. [DOI] [PubMed] [Google Scholar]
  15. KAISER A. D. Mutations in a temperate bacteriophage affecting its ability to lysogenize Escherichia coli. Virology. 1957 Feb;3(1):42–61. doi: 10.1016/0042-6822(57)90022-3. [DOI] [PubMed] [Google Scholar]
  16. KARLSSON U., KOORAJIAN S., ZABIN I., SJOESTRAND F. S., MILLER A. HIGH RESOLUTION ELECTRON MICROSCOPY ON HIGHLY PURIFIED BETA-GALACTOSIDASE FROM ESCHERICHIA COLI. J Ultrastruct Res. 1964 Jun;10:457–469. doi: 10.1016/s0022-5320(64)80022-8. [DOI] [PubMed] [Google Scholar]
  17. LOEB T. Isolation of a bacteriophage specific for the F plus and Hfr mating types of Escherichia coli K-12. Science. 1960 Mar 25;131(3404):932–933. doi: 10.1126/science.131.3404.932. [DOI] [PubMed] [Google Scholar]
  18. LURIA S. E., BURROUS J. W. Hybridization between Escherichia coli and Shigella. J Bacteriol. 1957 Oct;74(4):461–476. doi: 10.1128/jb.74.4.461-476.1957. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. MONOD J., COHEN-BAZIRE G., COHN M. Sur la biosynthèse de la beta-galactosidase (lactase) chez Escherichia coli; la spécificité de l'induction. Biochim Biophys Acta. 1951 Nov;7(4):585–599. doi: 10.1016/0006-3002(51)90072-8. [DOI] [PubMed] [Google Scholar]
  20. SILVER S. D., MOODY E. E., CLOWES R. C. LIMITS ON MATERIAL TRANSFER DURING F+ X F- MATINGS IN ESCHERICHIA COLI K12. J Mol Biol. 1965 May;12:283–286. doi: 10.1016/s0022-2836(65)80300-x. [DOI] [PubMed] [Google Scholar]
  21. SILVER S. D. The transfer of material during mating in Escherichia coli. Transfer of DNA and upper limits on the transfer of RNA and protein. J Mol Biol. 1963 May;6:349–360. doi: 10.1016/s0022-2836(63)80048-0. [DOI] [PubMed] [Google Scholar]
  22. SUSSMAN R., JACOB F. [On a thermosensitive repression system in the Escherichia coli lambda bacteriophage]. C R Hebd Seances Acad Sci. 1962 Feb 19;254:1517–1519. [PubMed] [Google Scholar]

Articles from Journal of Bacteriology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES