Skip to main content
PMC home page
Journal of Bacteriology logoLink to Journal of Bacteriology
. 1967 Dec;94(6):1807–1810. doi: 10.1128/jb.94.6.1807-1810.1967

Identification of Mycoplasma and Other Microorganisms by Polyacrylamide-Gel Electrophoresis of Cell Proteins

Shmuel Razin 1, Shlomo Rottem 1
PMCID: PMC276908  PMID: 4864403

Abstract

The proteins of Mycoplasma cells of various species produce highly reproducible and species-specific electrophoretic patterns in polyacrylamide gels containing 5 m urea and 35% acetic acid. These electrophoretic patterns can be used for the rapid identification and classification of Mycoplasma. Preliminary results indicate that this method may also be used for the identification and classification of other microorganisms.

Full text

PDF
1809

Images in this article

1808Image
on p.1808
1809Image
on p.1809
1809Image
on p.1809
1809Image
on p.1809

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. CHANOCK R. M., HAYFLICK L., BARILE M. F. Growth on artificial medium of an agent associated with atypical pneumonia and its identification as a PPLO. Proc Natl Acad Sci U S A. 1962 Jan 15;48:41–49. doi: 10.1073/pnas.48.1.41. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. De Ley J., Park I. W., Tijtgat R., Van Ermengem J. DNA homology and taxonomy of Pseudomonas and Xanthomonas. J Gen Microbiol. 1966 Jan;42(1):43–56. doi: 10.1099/00221287-42-1-43. [DOI] [PubMed] [Google Scholar]
  3. EDWARD D. G., FITZGERALD W. A. The isolation of organisms of the pleuropneumonia group from dogs. J Gen Microbiol. 1951 Aug;5(3):566–575. doi: 10.1099/00221287-5-3-566. [DOI] [PubMed] [Google Scholar]
  4. EDWARD D. G., FREUNDT E. A. The classification and nomenclature of organisms of the pleuropneumonia group. J Gen Microbiol. 1956 Feb;14(1):197–207. doi: 10.1099/00221287-14-1-197. [DOI] [PubMed] [Google Scholar]
  5. Edward D. G., Freundt E. A., Chanock R. M., Fabricant J., Hayflick L., Lemcke R. M., Rezin S., Somerson N. L., Wittler R. G. Recommendations on nomenclature of the order Mycoplasmatales. Science. 1967 Mar 31;155(3770):1694–1696. doi: 10.1126/science.155.3770.1694. [DOI] [PubMed] [Google Scholar]
  6. FALLON R. J., GRIST N. R., INMAN D. R., LEMCKE R. M., NEGRONI G., WOODS D. A. Further studies of agents isolated from tissue cultures inoculated with human leukaemic bone-marrow. Br Med J. 1965 Aug 14;2(5458):388–391. [PMC free article] [PubMed] [Google Scholar]
  7. GIRARDI A. J., HAYFLICK L., LEWIS A. M., SOMERSON N. L. RECOVERY OF MYCOPLASMAS IN THE STUDY OF HUMAN LEUKAEMIA AND OTHER MALIGNANCIES. Nature. 1965 Jan 9;205:188–189. doi: 10.1038/205188a0. [DOI] [PubMed] [Google Scholar]
  8. Korn E. D. Structure of biological membranes. Science. 1966 Sep 23;153(3743):1491–1498. doi: 10.1126/science.153.3743.1491. [DOI] [PubMed] [Google Scholar]
  9. LEMCKE R. M. A SEROLOGICAL COMPARISON OF VARIOUS SPECIES OF MYCOPLASMA BY AN AGAR GEL DOUBLE-DIFFUSION TECHNIQUE. J Gen Microbiol. 1965 Jan;38:91–100. doi: 10.1099/00221287-38-1-91. [DOI] [PubMed] [Google Scholar]
  10. LEMCKE R. M. THE SEROLOGICAL DIFFERENTIATION OF MYCOPLASMA STRAINS (PLEURO-PNEUMONIA-LIKE ORGANISMS) FROM VARIOUS SOURCES. J Hyg (Lond) 1964 Jun;62:199–219. doi: 10.1017/s0022172400039930. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  12. Leach R. H., Butler M. Compraison of mycoplasmas associated with human tumors, leukemia, and tissue cultures. J Bacteriol. 1966 Mar;91(3):934–941. doi: 10.1128/jb.91.3.934-941.1966. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. NEGRONI G. ISOLATION OF VIRUSES FROM LEUKAEMIC PATIENTS. Br Med J. 1964 Apr 11;1(5388):927–929. doi: 10.1136/bmj.1.5388.927. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Rottem S., Razin S. Electrophoretic patterns of membrane proteins of Mycoplasma. J Bacteriol. 1967 Aug;94(2):359–364. doi: 10.1128/jb.94.2.359-364.1967. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Somerson N. L., Reich P. R., Chanock R. M., Weissman S. M. Genetic differentiation by nucleic acid homology. 3. Relationships among mycoplasma, L-forms, and bacteria. Ann N Y Acad Sci. 1967 Jul 28;143(1):9–20. doi: 10.1111/j.1749-6632.1967.tb27638.x. [DOI] [PubMed] [Google Scholar]
  16. Takayama K., MacLennan D. H., Tzagoloff A., Stoner C. D. Studies on the electron transfer system. LXVII. Polyacrylamide gel electrophoresis of the mitochondrial electron transfer complexes. Arch Biochem Biophys. 1966 Apr;114(1):223–230. doi: 10.1016/0003-9861(66)90324-9. [DOI] [PubMed] [Google Scholar]

Articles from Journal of Bacteriology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES