Abstract
Ribonucleic acid synthesis was measured during the morphogenesis of Myxococcus xanthus. After induction of microcyst formation by the addition of glycerol to an exponential culture, net ribonucleic acid (RNA) synthesis was immediately terminated (measured either chemically or by the accumulation of acid-insoluble radioactivity). Extensive RNA turnover did take place, however, including RNA made both before and after induction. Sucrose gradient centrifugation revealed that ribosomes and ribosomal RNA were synthesized during microcyst formation even though there was no net RNA synthesis. Base analyses of the total RNA of vegetative cells and 120-min microcysts were indistinguishable.
Full text
PDF
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- DWORKIN M., GIBSON S. M. A SYSTEM FOR STUDYING MICROBIAL MORPHOGENESIS: RAPID FORMATION OF MICROCYSTS IN MYXOCOCCUS XANTHUS. Science. 1964 Oct 9;146(3641):243–244. doi: 10.1126/science.146.3641.243. [DOI] [PubMed] [Google Scholar]
- DWORKIN M. Nutritional requirements for vegetative growth of Myxococcus xanthus. J Bacteriol. 1962 Aug;84:250–257. doi: 10.1128/jb.84.2.250-257.1962. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Dworkin M., Sadler W. Induction of cellular morphogenesis in Myxococcus xanthus. I. General description. J Bacteriol. 1966 Apr;91(4):1516–1519. doi: 10.1128/jb.91.4.1516-1519.1966. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Rosenberg E., Katarski M., Gottlieb P. Deoxyribonucleic acid synthesis during exponential growth and microcyst formation in Myxococcus xanthus. J Bacteriol. 1967 Apr;93(4):1402–1408. doi: 10.1128/jb.93.4.1402-1408.1967. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Sadler W., Dworkin M. Induction of cellular morphogenesis in Myxococcus xanthus. II. Macromolecular synthesis and mechanism of inducer action. J Bacteriol. 1966 Apr;91(4):1520–1525. doi: 10.1128/jb.91.4.1520-1525.1966. [DOI] [PMC free article] [PubMed] [Google Scholar]