Abstract
Donnellan, J. Edward, Jr. (U.S. Army Natick Laboratories, Natick, Mass.), Ella H. Nags, and Hillel S. Levinson. Chemically defined, synthetic media for sporulation and for germination and growth of Bacillus subtilis. J. Bacteriol. 87:332–336. 1964.—From 90 to 130 mg (dry weight) of spores (about 1% dark forms) were obtained (per liter) from a chemically defined, synthetic medium, with a two-phase (polyethylene glycol-potassium phosphate) harvest procedure. Optimal sporulation occurred when glucose and glutamic acid were at a concentration of 10 mm in the medium. Ca++ and Mn++ were required for sporulation. Heat resistance, dipicolinic acid content, and properties of germination and postgerminative development of spores grown in different concentrations of Ca++ were investigated. Heat shock did not increase germination of spores derived from the synthetic medium. A synthetic medium, in which spore germination, emergence, and first cell division approached synchrony, was devised.
Full text
PDF
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- CHARNEY J., FISHER W. P., HEGARTY C. P. Managanese as an essential element for sporulation in the genus Bacillus. J Bacteriol. 1951 Aug;62(2):145–148. doi: 10.1128/jb.62.2.145-148.1951. [DOI] [PMC free article] [PubMed] [Google Scholar]
- DEMAIN A. L. Minimal media for quantitative studies with Bacillus subtilis. J Bacteriol. 1958 May;75(5):517–522. doi: 10.1128/jb.75.5.517-522.1958. [DOI] [PMC free article] [PubMed] [Google Scholar]
- DONNELLAN J. E., Jr, MOROWITZ H. J. The effect of charged particle irradiation on germination of Bacillus subtilis. Radiat Res. 1960 Jan;12:67–78. [PubMed] [Google Scholar]
- FOSTER J. W. Morphogenesis in bacteria: some aspects of spore formation. Q Rev Biol. 1956 Jun;31(2):102–118. doi: 10.1086/401259. [DOI] [PubMed] [Google Scholar]
- Foster J. W., Heiligman F. MINERAL DEFICIENCIES IN COMPLEX ORGANIC MEDIA AS LIMITING FACTORS IN THE SPORULATION OF AEROBIC BACILLI. J Bacteriol. 1949 Jun;57(6):613–615. doi: 10.1128/jb.57.6.613-615.1949. [DOI] [PMC free article] [PubMed] [Google Scholar]
- JANSSEN F. W., LUND A. J., ANDERSON L. E. Colorimetric assay for dipicolinic acid in bacterial spores. Science. 1958 Jan 3;127(3288):26–27. doi: 10.1126/science.127.3288.26. [DOI] [PubMed] [Google Scholar]
- KRASK B. J. Methionine sulfoxide and specific inhibition of sporulation in Bacillus subtilis. J Bacteriol. 1953 Sep;66(3):374–374. doi: 10.1128/jb.66.3.374-374.1953. [DOI] [PMC free article] [PubMed] [Google Scholar]
- LEVINSON H. S., SEVAG M. G. Stimulation of germination and respiration of the spores of Bacillus megatherium by manganese and monovalent anions. J Gen Physiol. 1953 May;36(5):617–629. doi: 10.1085/jgp.36.5.617. [DOI] [PMC free article] [PubMed] [Google Scholar]
- POWELL J. F. Factors affecting the germination of thick suspensions of bacillus subtilis spores in L-alanine solution. J Gen Microbiol. 1950 Sep;4(3):330–338. doi: 10.1099/00221287-4-3-330. [DOI] [PubMed] [Google Scholar]
- SACKS L. E., ALDERTON G. Behavior of bacterial spores in aqueous polymer two-phase systems. J Bacteriol. 1961 Sep;82:331–341. doi: 10.1128/jb.82.3.331-341.1961. [DOI] [PMC free article] [PubMed] [Google Scholar]
- SLEPECKY R., FOSTER J. W. Alterations in metal content of spores of Bacillus megaterium and the effect on some spore properties. J Bacteriol. 1959 Jul;78(1):117–123. doi: 10.1128/jb.78.1.117-123.1959. [DOI] [PMC free article] [PubMed] [Google Scholar]
- SUGIYAMA H. Studies on factors affecting the heat resistance of spores of Clostridium botulinum. J Bacteriol. 1951 Jul;62(1):81–96. doi: 10.1128/jb.62.1.81-96.1951. [DOI] [PMC free article] [PubMed] [Google Scholar]