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. 1964 Mar;87(3):536–542. doi: 10.1128/jb.87.3.536-542.1964

RESISTANCE AND CROSS-RESISTANCE OF ESCHERICHIA COLI S MUTANTS TO THE RADIOMIMETIC AGENT PROFLAVINE

Pearl Woody-Karrer 1, Joseph Greenberg 1
PMCID: PMC277050  PMID: 14129667

Abstract

Woody-Karrer, Pearl (Palo Alto Medical Research Foundation, Palo Alto, Calif.), and Joseph Greenberg. Resistance and cross-resistance of Escherichia coli S mutants to the radiomimetic agent proflavine. J. Bacteriol. 87:536–542. 1964.—All 50 of the first-step mutants of Escherichia coli S selected for resistance to proflavine were resistant to ultraviolet light and each of five different radiomimetic chemicals. The mutants were classified into eight types on the basis of their relative resistance to six different radiomimetic drugs and on the basis of the shape of their ultraviolet survival curves. Three of these types are identical to types previously isolated with other radiomimetic drugs; five of the types are new. A high proportion of the clones surviving proflavine treatment were phenotypically but not genetically resistant, and no strains were isolated which were resistant to proflavine but were not resistant to radiation.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. ADLER H. I., COPELAND J. C. Genetic analysis of radiation response in Escherichia coli. Genetics. 1962 Jun;47:701–712. doi: 10.1093/genetics/47.6.701. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. ALPER T., GILLIES N. E. The relationship between growth and survival after irradiation of Escherichia coli strain B and two resistant mutants. J Gen Microbiol. 1960 Feb;22:113–128. doi: 10.1099/00221287-22-1-113. [DOI] [PubMed] [Google Scholar]
  3. Brookes P., Lawley P. D. The reaction of mono- and di-functional alkylating agents with nucleic acids. Biochem J. 1961 Sep;80(3):496–503. doi: 10.1042/bj0800496. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. DE MARS R. I., LURIA S. E., FISHER H., LEVINTHAL C. The production of incomplete bacteriophage particles by the action of proflavine and the properties of the incomplete particles. Ann Inst Pasteur (Paris) 1953 Jan;84(1):113–128. [PubMed] [Google Scholar]
  5. GREENBERG J., MANDELL J. D., WOODY P. L. A preliminary report--resistance and cross-resistance of Escherichia coli mutants to radiomimetic agents. Cancer Chemother Rep. 1961 Apr;11:51–56. [PubMed] [Google Scholar]
  6. GREENBERG J., MANDELL J. D., WOODY P. L. Resistance and cross-resistance of Escherichia coli mutants to antitumour agent mitomycin C. J Gen Microbiol. 1961 Nov;26:509–520. doi: 10.1099/00221287-26-3-509. [DOI] [PubMed] [Google Scholar]
  7. HILL R. F. A radiation-sensitive mutant of Escherichia coli. Biochim Biophys Acta. 1958 Dec;30(3):636–637. doi: 10.1016/0006-3002(58)90112-4. [DOI] [PubMed] [Google Scholar]
  8. HILL R. F., SIMSON E. A study of radiosensitive and radioresistant mutants of Escherichia coli strain B. J Gen Microbiol. 1961 Jan;24:1–14. doi: 10.1099/00221287-24-1-1. [DOI] [PubMed] [Google Scholar]
  9. HOWARD-FLANDERS P., BOYCE R. P., SIMSON E., THERIOT L. A genetic locus in E. coli K12 that controls the reactivation of UV-photoproducts associated with thymine in DNA. Proc Natl Acad Sci U S A. 1962 Dec 15;48:2109–2115. doi: 10.1073/pnas.48.12.2109. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. LERMAN L. S. Structural considerations in the interaction of DNA and acridines. J Mol Biol. 1961 Feb;3:18–30. doi: 10.1016/s0022-2836(61)80004-1. [DOI] [PubMed] [Google Scholar]
  11. LERMAN L. S. The structure of the DNA-acridine complex. Proc Natl Acad Sci U S A. 1963 Jan 15;49:94–102. doi: 10.1073/pnas.49.1.94. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. LOVELESS L. E., SPOERL E., WEISMAN T. H. A survey of effects of chemicals on division and growth of yeast and Escherichia coli. J Bacteriol. 1954 Dec;68(6):637–644. doi: 10.1128/jb.68.6.637-644.1954. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. LUZZATI V., MASSON F., LERMAN L. S. Interaction of DNA and proflavine: a small-angle x-ray scattering study. J Mol Biol. 1961 Oct;3:634–639. doi: 10.1016/s0022-2836(61)80026-0. [DOI] [PubMed] [Google Scholar]
  14. MANDELL J. D., WOODY P. L., GREENBERG J. Resistance and cross-resistance of Escherichia coli mutants to anticancer agents. 1-Methyl-3-nitro-1-nitrosoguanidine. J Bacteriol. 1961 Mar;81:419–424. doi: 10.1128/jb.81.3.419-424.1961. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. RORSCH A., EDELMAN A., COHEN J. A. The gene-controlled radiation sensitivity in Escherichia coli. Biochim Biophys Acta. 1963 Feb 26;68:263–270. doi: 10.1016/0006-3002(63)90141-0. [DOI] [PubMed] [Google Scholar]
  16. RORSCH A., EDELMAN A., van der KAMP, COHEN J. A. Phenotypic and genotypic characterization of radiation sensitivity in Escherichia coli B. Biochim Biophys Acta. 1962 Aug 20;61:278–289. doi: 10.1016/0926-6550(62)90090-7. [DOI] [PubMed] [Google Scholar]
  17. SZYBALSKI W., BRYSON V. Genetic studies on microbial cross resistance to toxic agents. I. Cross resistance of Escherichia coli to fifteen antibiotics. J Bacteriol. 1952 Oct;64(4):489–499. doi: 10.1128/jb.64.4.489-499.1952. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. WOODY-KARRER P., GREENBERG J. RESISTANCE AND CROSS RESISTANCE OF ESCHERICHIA COLI S MUTANTS TO THE RADIOMIMETIC AGENT NITROFURAZONE. J Bacteriol. 1963 Jun;85:1208–1216. doi: 10.1128/jb.85.6.1208-1216.1963. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. WOODY P. L., MANDELL J. D., GREENBERG J. Resistance and cross-resistance of Escherichia coli mutants to anticancer agents: nitrogen mustard and nitromin. Radiat Res. 1961 Sep;15:290–297. [PubMed] [Google Scholar]
  20. Witkin E. M. Genetics of Resistance to Radiation in ESCHERICHIA COLI. Genetics. 1947 May;32(3):221–248. doi: 10.1093/genetics/32.3.221. [DOI] [PMC free article] [PubMed] [Google Scholar]

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