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. 1964 Mar;87(3):598–605. doi: 10.1128/jb.87.3.598-605.1964

ALTERATIONS IN THE MOUSE VIRULENCE OF SALMONELLA TYPHIMURIUM BY GENETIC RECOMBINATION

V Krishnapillai a,1, L S Baron a
PMCID: PMC277060  PMID: 14127576

Abstract

Krishnapillai, V. (Walter Reed Army Institute of Research, Washington, D.C.), and L. S. Baron. Alterations in the mouse virulence of Salmonella typhimurium by genetic recombination. J. Bacteriol. 87:598–605. 1964.—The genetic basis of mouse virulence was investigated with an avirulent strain of Salmonella abony as chromosomal donor and a virulent strain of S. typhimurium as recipient in recombination experiments. In these genetic crosses, the transfer of partial avirulence was found to segregate among the hybrids that were examined. At least two determinants controlling avirulence were depicted to account for the partial avirulence of the hybrids. One of these determinants is indicated as being in the region of the locus for streptomycin sensitivity or resistance, and the other was adjacent to the locus for inositol utilization. Moreover, both determinants were essential for the phenotypic expression of complete avirulence in a hybrid. This was established by the results of experiments in which an initial, partially avirulent hybrid was backcrossed with the S. abony donor so that it further received the additional determinant.

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Selected References

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  1. ADELBERG E. A., MYERS J. W. Modification of the penicillin technique for the selection of auxotrophic bacteria. J Bacteriol. 1953 Mar;65(3):348–353. doi: 10.1128/jb.65.3.348-353.1953. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. BACON G. A., BURROWS T. W., YATES M. The effects of biochemical mutation on the virulence of Bacterium typhosum; the loss of virulence of certain mutants. Br J Exp Pathol. 1951 Apr;32(2):85–96. [PMC free article] [PubMed] [Google Scholar]
  3. BARON L. S., SPILMAN W. M., CAREY W. F. Diploid heterozygous hybrids from matings between Escherichia coli and Salmonella typhosa. J Exp Med. 1960 Aug 1;112:361–372. doi: 10.1084/jem.112.2.361. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Baron L. S., Carey W. F., Spilman W. M. GENETIC RECOMBINATION BETWEEN ESCHERICHIA COLI AND SALMONELLA TYPHIMURIUM. Proc Natl Acad Sci U S A. 1959 Jul;45(7):976–984. doi: 10.1073/pnas.45.7.976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. FALKOW S., SCHNEIDER H., BARON L. S., FORMAL S. B. VIRULENCE OF ESCHERICHIA-SHIGELLA GENETIC HYBRIDS FOR THE GUINEA PIG. J Bacteriol. 1963 Dec;86:1251–1258. doi: 10.1128/jb.86.6.1251-1258.1963. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. FURNESS G., ROWLEY D. Transduction of virulence within the species Salmonella typhimurium. J Gen Microbiol. 1956 Aug;15(1):140–145. doi: 10.1099/00221287-15-1-140. [DOI] [PubMed] [Google Scholar]
  7. Falkow S., Rownd R., Baron L. S. GENETIC HOMOLOGY BETWEEN ESCHERICHIA COLI K-12 AND SALMONELLA. J Bacteriol. 1962 Dec;84(6):1303–1312. doi: 10.1128/jb.84.6.1303-1312.1962. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. LEDERBERG J., LEDERBERG E. M. Replica plating and indirect selection of bacterial mutants. J Bacteriol. 1952 Mar;63(3):399–406. doi: 10.1128/jb.63.3.399-406.1952. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. LOVELESS A., HOWARTH S. Mutation of bacteria at high levels of survival by ethyl methane sulphonate. Nature. 1959 Dec 5;184:1780–1782. doi: 10.1038/1841780a0. [DOI] [PubMed] [Google Scholar]
  10. MAKELA P. H. HFR males in Salmonella abony. Genetics. 1963 Mar;48:423–429. doi: 10.1093/genetics/48.3.423. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. MIYAKE T., DEMEREC M. Salmonella Escherichia hybrids. Nature. 1959 Jun 6;183(4675):1586–1586. doi: 10.1038/1831586a0. [DOI] [PubMed] [Google Scholar]
  12. Miyake T. Exchange of Genetic Material between Salmonella Typhimurium and Escherichia Coli K-12. Genetics. 1962 Aug;47(8):1043–1052. doi: 10.1093/genetics/47.8.1043. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. SMITH S. M., STOCKER B. A. Colicinogeny and recombination. Br Med Bull. 1962 Jan;18:46–51. doi: 10.1093/oxfordjournals.bmb.a069934. [DOI] [PubMed] [Google Scholar]
  14. ZINDER N. D., LEDERBERG J. Genetic exchange in Salmonella. J Bacteriol. 1952 Nov;64(5):679–699. doi: 10.1128/jb.64.5.679-699.1952. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. ZINDER N. D. Sexuality and mating in salmonella. Science. 1960 Mar 25;131(3404):924–926. doi: 10.1126/science.131.3404.924. [DOI] [PubMed] [Google Scholar]

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