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. 1964 May;87(5):1027–1033. doi: 10.1128/jb.87.5.1027-1033.1964

CONTROL OF ASPARTATE TRANSCARBAMYLASE ACTIVITY IN TYPE 5 ADENOVIRUS-INFECTED HELA CELLS

Richard A Consigli a,1, Harold S Ginsberg a
PMCID: PMC277141  PMID: 5874531

Abstract

Consigli, Richard A. (University of Pennsylvania, Philadelphia), and Harold S. Ginsberg. Control of aspartate transcarbamylase activity in type 5 adenovirus-infected HeLa cells. J. Bacteriol. 87:1027–1033. 1964.—Type 5 adenovirus infection induces increased aspartate transcarbamylase (ATCase) activity during the period of magnified nucleic acid biosynthesis. Increased activity can be prevented by addition of pyrimidines to the culture medium. ATCase in HeLa cells is regulated by feedback inhibition, and purified enzyme can be inhibited in vitro by cytidine triphosphate (CTP). The enzyme from infected cells has a pH optimum, maximal velocity, and Km for aspartate distinctly different from ATCase from control cells. However, heating of ATCase from uninfected cells converts the enzyme so that its characteristics are identical with enzyme from infected cells. Conversely, addition of CTP to ATCase from infected cells changes the characteristics of the enzyme so that they are the same as those of enzyme from uninfected cells. The evidence presented suggests that increased nucleic acid biosynthesis in infected cells initiates a release from feedback inhibition and increases ATCase activity by reducing the concentration of pyrimidines and purines in the acid-soluble pool.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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