Abstract
Lones, George W. (National Institute of Allergy and Infectious Diseases, U.S. Public Health Service, Bethesda, Md.), and Carl Peacock. Metabolism of mannitol by Coccidioides immitis. J. Bacteriol. 87:1114–1117. 1964.—Strain M-11 of Coccidioides immitis was found to utilize mannitol for growth in the mycelial form but not in the spherule form. Cell-free extracts of both forms, grown on glucose, were capable of reducing nicotinamide adenine dinucleotide with mannitol-1-PO4 but not with mannitol. The extracts accomplished a rapid oxidation of reduced nicotinamide adenine dinucleotide by fructose-6-PO4, the expected product of mannitol-1-PO4 oxidation. Fructose was inactive. Paper electrophoresis and chromatography with several solvent systems demonstrated a substance in extracts of both mycelium and spherules having a migration consistent with that of mannitol.
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Selected References
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