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. 1964 Jun;87(6):1383–1388. doi: 10.1128/jb.87.6.1383-1388.1964

COMPARISON OF CYTOCHROMES IN MYCOBACTERIA GROWN IN VITRO AND IN VIVO

Takashi Kusaka 1,2, Ryo Sato 1,2, Ko Shoji 1,2
PMCID: PMC277214  PMID: 14188717

Abstract

Kusaka, Takashi (National Institute for Leprosy Research, Tokyo, Japan), Ryo Sato, and Ko Shoji. Comparison of cytochromes in mycobacteria grown in vitro and in vivo. J. Bacteriol. 87:1383–1388. 1964.—Spectrophotometric investigations were made on cell suspensions or particulate fractions of four species of mycobacteria which had been cultivated in vitro. The results obtained indicated the presence in Mycobacterium smegmatis of cytochromes of the a, b1, and c types, as well as a CO-binding pigment similar to cytochrome o; in BCG and M. avium, cytochromes of the a, b, and c types were present; and, in M. paratuberculosis, cytochromes of the a, b1, and c types were present. Although no clear interrelations were evident between the cytochrome patterns of these organisms and the ease with which they can be cultivated in vitro, it was found that the total reduced nicotinamide adenine dinucleotide oxidase activity of the particulate fraction was remarkably low in M. paratuberculosis, which can be grown in vitro only with great difficulty. The cells of BCG grown in vivo and those of M. lepraemurium isolated from leprous nodules of mice were found to be completely deficient in cytochrome pigments.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. ANDREJEW A., GERNEZ-RIEUX C., TACQUET A. Cytochromes et activité cytochrome-oxydasique des Mycobactéries sensibles et résistantes à l'INH. Ann Inst Pasteur (Paris) 1957 Sep;93(3):281–288. [PubMed] [Google Scholar]
  2. AOYAGI T., MIZUNO D. Cultivation of mycobacteria rocked in non-protein medium containing a high concentration of tween 80. J Gen Microbiol. 1959 Apr;20(2):173–179. doi: 10.1099/00221287-20-2-173. [DOI] [PubMed] [Google Scholar]
  3. BASTARRACHEA F., GOLDMAN D. S. Diphosphopyridine nucleotide-linked nitrate reductase from Mycobacterium tuberculosis. Biochim Biophys Acta. 1961 Jun 10;50:174–176. doi: 10.1016/0006-3002(61)91077-0. [DOI] [PubMed] [Google Scholar]
  4. BLOCH H., SEGAL W. Biochemical differentiation of Mycobacterium tuberculosis grown in vivo and in vitro. J Bacteriol. 1956 Aug;72(2):132–141. doi: 10.1128/jb.72.2.132-141.1956. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. CASTOR L. N., CHANCE B. Photochemical determinations of the oxidases of bacteria. J Biol Chem. 1959 Jun;234(6):1587–1592. [PubMed] [Google Scholar]
  6. CHANCE B. The carbon monoxide compounds of the cytochrome oxidases. I. Difference spectra. J Biol Chem. 1953 May;202(1):383–396. [PubMed] [Google Scholar]
  7. GOLDMAN D. S., WAGNER M. J., ODA T., SHUG A. L. OXIDATION OF REDUCED NICOTINAMIDE-ADENINE DINUCLEOTIDE BY SUBCELLULAR PARTICLES FROM MYCOBACTERIUM TUBERCULOSIS. Biochim Biophys Acta. 1963 Jul 9;73:367–379. doi: 10.1016/0006-3002(63)90438-4. [DOI] [PubMed] [Google Scholar]
  8. HANKS J. H., GRAY C. T. The metabolic properties of mycobacteria and the pathogenesis of mycobacterial disease. Bibl Tuberc. 1956;(10):1–16. [PubMed] [Google Scholar]
  9. KUSAKA T. Studies on the metabolism of murine leprosy bacilli. I. Stimulating effect of mitochondria extract of rat liver on the metabolism of bacilli. Jpn J Med Sci Biol. 1958 Aug;11(4):277–285. doi: 10.7883/yoken1952.11.277. [DOI] [PubMed] [Google Scholar]
  10. LENHOFF H. M., NICHOLAS D. J., KAPLAN N. O. Effects of oxygen, iron, and molybdenum on routes of electron transfer in Pseudomonas fluorescens. J Biol Chem. 1956 Jun;220(2):983–995. [PubMed] [Google Scholar]
  11. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  12. SMITH L. Bacterial cytochromes. Bacteriol Rev. 1954 Jun;18(2):106–130. doi: 10.1128/br.18.2.106-130.1954. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. SMITH L., WHITE D. C. Structure of the respiratory chain system as indicated by studies with Hemophilus parainfluenzae. J Biol Chem. 1962 Apr;237:1337–1341. [PubMed] [Google Scholar]
  14. TODD C. M. Occurrence of cytochrome and copro-porphyrin in mycobacteria. Biochem J. 1949;45(4):386–390. doi: 10.1042/bj0450386. [DOI] [PMC free article] [PubMed] [Google Scholar]

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