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. 1964 Sep;88(3):771–775. doi: 10.1128/jb.88.3.771-775.1964

GROWTH CHARACTERISTICS OF THE BLUE-GREEN ALGAL VIRUS LPP-1

Robert S Safferman 1, Mary-Ellen Morris 1
PMCID: PMC277376  PMID: 14208517

Abstract

Safferman, Robert S. (Robert A. Taft Sanitary Engineering Center, Cincinnati, Ohio), and Mary-Ellen Morris. Growth characteristics of the blue-green algal virus LPP- 1. J. Bacteriol. 88:771–775. 1964.—The blue-green algal (BGA) virus, strain LPP-1, formed two distinct plaque variants. During subsequent propagation, each of the isolated variants eventually reverted to a mixture of both plaque types. The large-plaque-former, multiplying at a somewhat faster rate, showed maximal virus production within 60 hr after infection; maximal titer of the small-plaque mutant was reached after a 76-hr incubation period. No differences were observed in either their host spectra or their pH and thermal stability. Evidence was presented which demonstrated that BGA virus samples could be assayed with reasonable accuracy from plaque counts. Variations resulting from this plating technique were within the range of experimental error that has been generally reported for such a system.

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Selected References

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  1. GROMAN N. B., LOCKART R. Z. A study of the application of standard phage techniques to the host-phage system of Corynebacterium diphtheriae. J Bacteriol. 1953 Aug;66(2):178–183. doi: 10.1128/jb.66.2.178-183.1953. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. LIEBHABER H., TAKEMOTO K. K. Alteration plaque morphology of EMC virus with polycations. Virology. 1961 Aug;14:502–504. doi: 10.1016/0042-6822(61)90349-x. [DOI] [PubMed] [Google Scholar]
  3. LURIA S. E., WILLIAMS R. C., BACKUS R. C. Electron micrographic counts of bacteriophage particles. J Bacteriol. 1951 Feb;61(2):179–188. doi: 10.1128/jb.61.2.179-188.1951. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. SAFFERMAN R. S., MORRIS M. E. Algal virus: isolation. Science. 1963 May 10;140(3567):679–680. doi: 10.1126/science.140.3567.679. [DOI] [PubMed] [Google Scholar]
  5. SCHNEIDER I. R., DIENER T. O., SAFFERMAN R. S. BLUE-GREEN ALGAL VIRUS LPP-1: PURIFICATION AND PARTIAL CHARACTERIZATION. Science. 1964 May 29;144(3622):1127–1130. doi: 10.1126/science.144.3622.1127. [DOI] [PubMed] [Google Scholar]
  6. TAKEMORI N., NOMURA S. Mutation of polioviruses with respect to size of plaque. II. Reverse mutation of minute plaque mutant. Virology. 1960 Oct;12:171–184. doi: 10.1016/0042-6822(60)90192-6. [DOI] [PubMed] [Google Scholar]
  7. TAKEMOTO K. K., LIEBHABER H. Virus-polysaccharide interactions. I. An agar polysaccharide determining plaque morphology of EMC virus. Virology. 1961 Aug;14:456–462. doi: 10.1016/0042-6822(61)90338-5. [DOI] [PubMed] [Google Scholar]

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