Skip to main content
PMC home page
Journal of Bacteriology logoLink to Journal of Bacteriology
. 1964 Nov;88(5):1205–1210. doi: 10.1128/jb.88.5.1205-1210.1964

METABOLIC ACTIVITY IN COXIELLA BURNETII

Richard A Ormsbee 1, Marius G Peacock 1
PMCID: PMC277395  PMID: 14234772

Abstract

Ormsbee, Richard A. (Rocky Mountain Laboratory, Hamilton, Mont.), and Marius G. Peacock. Metabolic activity in Coxiella burnetii. J. Bacteriol. 88:1205–1210. 1964.—Purified suspensions of Coxiella burnetii were shown to utilize α-ketoglutarate, succinate, fumarate, malate, oxaloacetate, pyruvate, glutamate, and serine. The addition of nicotinamide adenine dinucleotide+ was necessary to elicit the maximal rate of oxygen uptake with l-glutamate as substrate, but was unnecessary when other substrates were employed. It was concluded that the Krebs cycle of intermediary carbohydrate metabolism probably operates within C. burnetii, and that pyruvate is the chief energy source.

Full text

PDF
1206

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. BELL E. J., PHILIP C. B. The human rickettsioses. Annu Rev Microbiol. 1952;6:91–118. doi: 10.1146/annurev.mi.06.100152.000515. [DOI] [PubMed] [Google Scholar]
  2. BOVARNICK M. R., MILLER J. C. Oxidation and transamination of glutamate by typhus rickettsiae. J Biol Chem. 1950 Jun;184(2):661–676. [PubMed] [Google Scholar]
  3. BOVARNICK M. R., MILLER J. C., SNYDER J. C. The influence of certain salts, amino acids, sugars, and proteins on the stability of rickettsiae. J Bacteriol. 1950 Apr;59(4):509–522. doi: 10.1128/jb.59.4.509-522.1950. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. CONSIGLI R. A., PARETSKY D. Oxidation of glucose 6-phosphate and isocitrate by Coxiella burnetii. J Bacteriol. 1962 Jan;83:206–207. doi: 10.1128/jb.83.1.206-207.1962. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. DELWICHE E. A. Metabolism of microorganisms. Annu Rev Microbiol. 1955;9:145–172. doi: 10.1146/annurev.mi.09.100155.001045. [DOI] [PubMed] [Google Scholar]
  6. HAYES J. E., HAHN F. E., COHN Z. A., JACKSON E. B., SMADEL J. E. Metabolic studies of rickettsiae. IV. Terminal respiratory enzymes in Rickettsia mooseri. Biochim Biophys Acta. 1957 Dec;26(3):570–576. doi: 10.1016/0006-3002(57)90104-x. [DOI] [PubMed] [Google Scholar]
  7. KALNITSKY G. The non-enzymatic oxidation of alpha-ketoglutarate. I. The effects of manganous ions and amino acids. J Biol Chem. 1953 Apr;201(2):817–823. [PubMed] [Google Scholar]
  8. KARP A. An immunological purification of typhus Rickettsiae. J Bacteriol. 1954 Apr;67(4):450–455. doi: 10.1128/jb.67.4.450-455.1954. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. KREBS H. A. The tricarboxylic acid cycle. Harvey Lect. 1948 1949;Series 44:165–199. [PubMed] [Google Scholar]
  10. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  11. ORMSBEE R. A. A method of purifying Coxiella burnetii and other pathogenic Rickettsiae. J Immunol. 1962 Jan;88:100–108. [PubMed] [Google Scholar]
  12. ORMSBEE R. A. AN AGGLUTINATION-RESUSPENSION TEST FOR Q FEVER ANTIBODIES. J Immunol. 1964 Jan;92:159–166. [PubMed] [Google Scholar]
  13. ORMSBEE R. A., WEISS E. TRACHOMA AGENT: GLUCOSE UTILIZATION BY PURIFIED SUSPENSIONS. Science. 1963 Nov 22;142(3595):1077–1078. doi: 10.1126/science.142.3595.1077. [DOI] [PubMed] [Google Scholar]
  14. PARETSKY D., DOWNS C. M., CONSIGLI R. A., JOYCE B. K. Studies on the physiology of rickettsiae. I. Some enzyme systems of Coxiella burnetii. J Infect Dis. 1958 Jul-Aug;103(1):6–11. doi: 10.1093/infdis/103.1.6. [DOI] [PubMed] [Google Scholar]
  15. PIZZI M. Sampling variation of the fifty percent end-point, determined by the Reed-Muench (Behrens) method. Hum Biol. 1950 Sep;22(3):151–190. [PubMed] [Google Scholar]
  16. PRICE W. H. A quantitative analysis of the factors involved in the variations in virulence of Rickettsiae. Science. 1953 Jul 10;118(3054):49–52. doi: 10.1126/science.118.3054.49. [DOI] [PubMed] [Google Scholar]
  17. WELSH H. H., JENSEN F. W., LENNETTE E. H. Q fever studies. XX. Comparison of four serologic techniques for the detection and measurement of antibody to Coxiella burnetii in naturally exposed sheep. Am J Hyg. 1959 Jul;70(1):1–13. [PubMed] [Google Scholar]
  18. WISSEMAN C. L., Jr, HAHN F. E., JACKSON E. B., BOZEMAN M. F., SMADEL J. E. Metabolic studies of rickettsiae. II. Studies on the pathway of glutamate oxidation by purified suspensions of Rickettsia mooseri. J Immunol. 1952 Mar;68(3):251–264. [PubMed] [Google Scholar]

Articles from Journal of Bacteriology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES