Abstract
Nishihara, Mutsuko (University of California, Los Angeles), and W. R. Romig. Temperature-sensitive mutants of Bacillus subtilis bacteriophage SP3. II. In vivo complementation studies. J. Bacteriol. 88:1230–1239. 1964.—A plate-spotting procedure was used in initial attempts to group the temperature-sensitive Bacillus subtilis phage SP3 mutants by complementation. The results obtained did not show any clear patterns of reactions among the mutants. Crosses were, therefore, repeated in broth at a temperature of 49 C, which greatly reduced the extent of replication of each mutant type alone. The data on mixed infections indicated that there was a minimum of six complementation groups. Of the 12 isolates, 7 did not seem to complement with each other; the rest complemented with each other and with the seven noncomplementing mutants. There was a positive correlation between the complementation reaction of a pair and the recovery of wild-phenotype phages from a 49 C broth lysate. The relative proportion of phages capable of forming wild-phenotype plaques on plates incubated at 46 C to the total number of plaque-forming units was higher in a lysate of a mixed infection with two mutants than in lysates of each mutant alone. Moreover, this frequency was higher for a mixed lysate made at 49 C than for a lysate of the same two mutants made at 37 C. These observations suggested that genetic recombination might occur at 49 C, and that the increased recovery of wild-phenotype phages in lysates made at this temperature might be due to a selective advantage for these phages. Recombination experiments at 37 C with some complementing pairs gave frequencies of 2.0 to 4.8%. The ratio of wild-phenotype revertants to total phages in the stock lysates used for these crosses at 37 C was less than 10−6. The noncomplementing mutants were not conclusively shown to be nonidentical.
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- CAMPBELL A., DELCAMPILLO-CAMPBELL A. MUTANT OF LAMBDA BACTERIOPHAGE PRODUCING A THERMOLABILE ENDOLYSIN. J Bacteriol. 1963 Jun;85:1202–1207. doi: 10.1128/jb.85.6.1202-1207.1963. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Chase M, Doermann A H. High Negative Interference over Short Segments of the Genetic Structure of Bacteriophage T4. Genetics. 1958 May;43(3):332–353. doi: 10.1093/genetics/43.3.332. [DOI] [PMC free article] [PubMed] [Google Scholar]
- DIRKSEN M. L., HUTSON J. C., BUCHANAN J. M. HOST-DEPENDENT SYNTHESIS OF ALTERED DEOXYCYTIDYLATE HYDROXYMETHYLASE AFTER INFECTION OF ESCHERICHIA COLI WITH CERTAIN AMBER MUTANTS OF BACTERIOPHAGE T4. Proc Natl Acad Sci U S A. 1963 Sep;50:507–513. doi: 10.1073/pnas.50.3.507. [DOI] [PMC free article] [PubMed] [Google Scholar]
- EDGAR R. S., FEYNMAN R. P., KLEIN S., LIELAUSIS I., STEINBERG C. M. Mapping experiments with r mutants of bacteriophage T4D. Genetics. 1962 Feb;47:179–186. doi: 10.1093/genetics/47.2.179. [DOI] [PMC free article] [PubMed] [Google Scholar]
- HELINSKI D. R., YANOFSKY C. Correspondence between genetic data and the position of amino acid alteration in a proein. Proc Natl Acad Sci U S A. 1962 Feb;48:173–183. doi: 10.1073/pnas.48.2.173. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Horowitz N. H., Fling M. Genetic Determination of Tyrosinase Thermostability in Neurospora. Genetics. 1953 Jul;38(4):360–374. doi: 10.1093/genetics/38.4.360. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Maas W. K., Davis B. D. Production of an Altered Pantothenate-Synthesizing Enzyme by a Temperature-Sensitive Mutant of Escherichia Coli. Proc Natl Acad Sci U S A. 1952 Sep;38(9):785–797. doi: 10.1073/pnas.38.9.785. [DOI] [PMC free article] [PubMed] [Google Scholar]
- NISHIHARA M., ROMIG W. R. TEMPERATURE-SENSTIVIE MUTANTS OF BACILLUS SUBTILIS BACTERIOPHAGE SP3. I. ISOLATION AND CHARACTERIZATION. J Bacteriol. 1964 Nov;88:1220–1229. doi: 10.1128/jb.88.5.1220-1229.1964. [DOI] [PMC free article] [PubMed] [Google Scholar]
- ROMIG W. R., BRODETSKY A. M. Isolation and preliminary characterization of bacteriophages for Bacillus subtilis. J Bacteriol. 1961 Jul;82:135–141. doi: 10.1128/jb.82.1.135-141.1961. [DOI] [PMC free article] [PubMed] [Google Scholar]
- STREISINGER G., MUKAI F., DREYER W. J., MILLER B., HORIUCHI S. Mutations affecting the lysozyme of phage T4. Cold Spring Harb Symp Quant Biol. 1961;26:25–30. doi: 10.1101/sqb.1961.026.01.007. [DOI] [PubMed] [Google Scholar]
- YANOFSKY C., HELINSKI D. R., MALING B. D. The effects of mutation on the composition and properties of the A protein of Escherichia coli tryptohan synthetase. Cold Spring Harb Symp Quant Biol. 1961;26:11–24. doi: 10.1101/sqb.1961.026.01.006. [DOI] [PubMed] [Google Scholar]