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. 1964 Dec;88(6):1618–1623. doi: 10.1128/jb.88.6.1618-1623.1964

DIFFERENTIATION OF PATHOGENIC AND SAPROPHYTIC LEPTOSPIRES WITH 8-AZAGUANINE

Russell C Johnson a,1, Palmer Rogers a
PMCID: PMC277463  PMID: 14244050

Abstract

Johnson, Russell C. (University of Minnesota, Minneapolis), and Palmer Rogers. Differentiation of pathogenic and saprophytic leptospires with 8-azaguanine. J. Bacteriol. 88:1618–1623. 1964.—The use of the purine analogue, 8-azaguanine, as a differential agent for the separation of pathogenic and saprophytic leptospires was investigated. Growth of strains of the saprophyte Leptospira biflexa was almost insensitive to the bacteriostatic action of 8-azaguanine at concentrations varying from 25 to 600 μg/ml; these saprophytic leptospires were serially transferred five times in media containing 225 μg without any change in growth rate or cell yield. In contrast, decreased growth rate and cell yield of the pathogenic serotypes were observed with 25 to 50 μg/ml of 8-azaguanine. Complete inhibition of growth occurred at concentrations of 100 μg/ml and above. A medium containing 225 μg/ml of 8-azaguanine was successfully used to differentiate 20 serotypes of pathogenic leptospires and 10 saprophytic strains. L. andaman CH11, L. semarang Veldrat S1 73, and L. andaman Correa, were classified with the L. biflexa strains on the basis of their growth response to 8-azaguanine.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. BABUDIERI B., DYMOWSKA Z. [Studies on a Polish strain of Leptospira of the serogroup "Semaranga"]. Zentralbl Bakteriol. 1961 May;182:129–134. [PubMed] [Google Scholar]
  2. FUZI M., CSOKA R. An egg-yolk reaction test for the differentiation of Leptospirae. J Pathol Bacteriol. 1961 Jul;82:208–212. doi: 10.1002/path.1700820129. [DOI] [PubMed] [Google Scholar]
  3. JOHNSON R. C., GARY N. D. NUTRITION OF LEPTOSPIRA POMONA. II. FATTY ACID REQUIREMENTS. J Bacteriol. 1963 May;85:976–982. doi: 10.1128/jb.85.5.976-982.1963. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. JOHNSON R. C., GARY N. D. Nutrition of Leptospira pomona. 1. A chemically defined substitute for rabbit serum ultrafiltrate. J Bacteriol. 1962 Mar;83:668–672. doi: 10.1128/jb.83.3.668-672.1962. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. JOHNSON R. C., ROGERS P. 5-FLUOROURACIL AS A SELECTIVE AGENT FOR GROWTH OF LEPTOSPIRAE. J Bacteriol. 1964 Feb;87:422–426. doi: 10.1128/jb.87.2.422-426.1964. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. JOHNSON R. C., ROGERS P. METABOLISM OF LEPTOSPIRAE. I. UTILIZATION OF AMINO ACIDS AND PURINE, AND PYRIMIDINE BASES. Arch Biochem Biophys. 1964 Sep;107:459–470. doi: 10.1016/0003-9861(64)90302-9. [DOI] [PubMed] [Google Scholar]
  7. JOHNSON R. C., WILSON J. B. Nutrition of Leptospira pomona. J Bacteriol. 1960 Sep;80:406–411. doi: 10.1128/jb.80.3.406-411.1960. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. SMITH J. D., MATTHEWS R. E. The metabolism of 8-azapurines. Biochem J. 1957 Jun;66(2):323–333. doi: 10.1042/bj0660323. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. YANAGAWA R., HIRAMUNE T., AKAIKE Y. GROWTH OF SAPROPHYTIC AND PATHOGENIC LEPTOSPIRAE ON SOLID MEDIUM IN CARBON DIOXIDE-FREE AIR. J Bacteriol. 1963 Apr;85:953–954. doi: 10.1128/jb.85.4.953-954.1963. [DOI] [PMC free article] [PubMed] [Google Scholar]

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