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. 1965 Mar;89(3):813–818. doi: 10.1128/jb.89.3.813-818.1965

Hemolysin of Mycoplasma pneumoniae

Norman L Somerson a, Robert H Purcell a, David Taylor-Robinson a,1, Robert M Chanock a
PMCID: PMC277542  PMID: 14273666

Abstract

Somerson, Norman L. (National Institute of Allergy and Infectious Diseases, Bethesda, Md.), Robert H. Purcell, David Taylor-Robinson, and Robert M. Chanock. Hemolysin of Mycoplasma pneumoniae. J. Bacteriol. 89:813–818. 1965.—Discrete colonies of Mycoplasma pneumoniae or M. laidlawii on agar produced complete (β) lysis of guinea pig erythrocytes. Crowding of colonies on agar plates, omission of yeast extract from the medium, and incubation under reduced oxygen tension inhibited hemolysin production. Colonies which were not viable after ultraviolet radiation or heating at 56 C did not produce hemolysin. In addition, hemolysis was suppressed in old cultures in which viability was diminished, and was eliminated when colonies were removed from the agar surface by micromanipulation. The hemolysin passed through a viscose dialysis membrane. These findings suggest that the hemolysin is labile, continuously released by the colonies, of low molecular weight, and probably nonprotein in nature.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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