Abstract
Gerwing, Julia (University of British Columbia, Vancouver, B.C., Canada), Claude E. Dolman, and Arthur Ko. Mechanism of tryptic activation of Clostridium botulinum type E toxin. J. Bacteriol. 89:1176–1179. 1965.—The toxic peptide of trypsin activated Clostridium botulinum type E toxin was purified by chromatography through columns packed with Sephadex G-75 and G-50. The molecular weight of the active peptide was estimated to lie between 10,000 and 12,000. Amino acid analyses indicated that the active peptide had lost at least 18 of the amino acid residues present in the original protein. The active peptide and the original protein were found to have different N-terminal amino acid residues. The mechanism of tryptic activation apparently involves chiefly the removal of amino acids from the N-terminus of the toxin molecule.
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Selected References
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- FRAENKEL-CONRAT H., HARRIS J. I., LEVY A. L. Recent developments in techniques for terminal and sequence studies in peptides and proteins. Methods Biochem Anal. 1955;2:359–425. doi: 10.1002/9780470110188.ch12. [DOI] [PubMed] [Google Scholar]
- GERWING J., DOLMAN C. E., ARNOTT D. A. Activation phenomenon of Clostridium botulinum type E toxin. J Bacteriol. 1962 Aug;84:302–306. doi: 10.1128/jb.84.2.302-306.1962. [DOI] [PMC free article] [PubMed] [Google Scholar]
- GERWING J., DOLMAN C. E., ARNOTT D. A. Purification and activation of Clostridium botulinum type E toxin. J Bacteriol. 1961 May;81:819–822. doi: 10.1128/jb.81.5.819-822.1961. [DOI] [PMC free article] [PubMed] [Google Scholar]
- GERWING J., DOLMAN C. E., REICHMANN M. E., BAINS H. S. PURIFICATION AND MOLECULAR WEIGHT DETERMINATION OF CLOSTRIDIUM BOTULINUM TYPE E TOXIN. J Bacteriol. 1964 Jul;88:216–219. doi: 10.1128/jb.88.1.216-219.1964. [DOI] [PMC free article] [PubMed] [Google Scholar]
- SAKAGUCHI G., SAKAGUCHI S. A simple method for purification of type E botulinal toxin from the precursor extract of the bacterial cells. Jpn J Med Sci Biol. 1961 Dec;14:243–243. doi: 10.7883/yoken1952.14.243. [DOI] [PubMed] [Google Scholar]
- SAKAGUCHI G., SAKAGUCHI S., IMAI N. COMPARATIVE GEL FILTRATION OF TOXIN PRECURSOR AND TRYPSIN-ACTIVATED TOXIN OF CLOSTRIDIUM BOTULINUM TYPE E. J Bacteriol. 1964 Feb;87:401–407. doi: 10.1128/jb.87.2.401-407.1964. [DOI] [PMC free article] [PubMed] [Google Scholar]
- SAKAGUCHI G., SAKAGUCHI S. Studies on toxin production of Clostridium botulinum type E. III. Characterization of toxin precursor. J Bacteriol. 1959 Jul;78(1):1–9. doi: 10.1128/jb.78.1.1-9.1959. [DOI] [PMC free article] [PubMed] [Google Scholar]