Skip to main content
PMC home page
Proceedings of the National Academy of Sciences of the United States of America logoLink to Proceedings of the National Academy of Sciences of the United States of America
. 1969 Feb;62(2):377–384. doi: 10.1073/pnas.62.2.377

CELL KINETIC STUDIES IN MIXED LEUKOCYTE CULTURES: AN in vitro MODEL OF HOMOGRAFT REACTIVITY*

Fritz H Bach 1,2,†, Harvey Bock 1,2, Kenneth Graupner 1,2, Elizabeth Day 1,2, Heidi Klostermann 1,2
PMCID: PMC277806  PMID: 5256217

Abstract

The recognition phase of homograft immunity can be studied in vitro in the mixed leukocyte culture reaction. In this reaction, at the end of seven days, up to 30 per cent of the lymphocytes in culture “respond” to a single allogeneic cell stimulus. This paper presents evidence that the lymphocytes responding in culture divide with the generation time of 18-21 hours, with some asynchrony, and that a large percentage of the cells found at the end of the culture period may be the products by division of a small number of cells initially responding. Two estimates are made of the frequency of the initially responding unit (the cell(s) responsible for initiating the response). These are probably minimum estimates and are in the range of 1 in 200 to 1 in 2000 cells which can initially respond. This very high frequency of responding units as compared with the responding units in immediate type hypersensitivity (antibody production) is discussed.

Full text

PDF
384

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Auerbach R., Globerson A. In vitro induction of the graft-versus-host reaction. Exp Cell Res. 1966 Apr;42(1):31–41. doi: 10.1016/0014-4827(66)90316-8. [DOI] [PubMed] [Google Scholar]
  2. BACH F., HIRSCHHORN K. LYMPHOCYTE INTERACTION: A POTENTIAL HISTOCOMPATIBILITY TEST IN VITRO. Science. 1964 Feb 21;143(3608):813–814. doi: 10.1126/science.143.3608.813. [DOI] [PubMed] [Google Scholar]
  3. BAIN B., VAS M. R., LOWENSTEIN L. THE DEVELOPMENT OF LARGE IMMATURE MONONUCLEAR CELLS IN MIXED LEUKOCYTE CULTURES. Blood. 1964 Jan;23:108–116. [PubMed] [Google Scholar]
  4. Bach F. H., Amos D. B. Hu-1: Major histocompatibility locus in man. Science. 1967 Jun 16;156(3781):1506–1508. doi: 10.1126/science.156.3781.1506. [DOI] [PubMed] [Google Scholar]
  5. Bach F. H., Voynow N. K. One-way stimulation in mixed leukocyte cultures. Science. 1966 Jul 29;153(3735):545–547. doi: 10.1126/science.153.3735.545. [DOI] [PubMed] [Google Scholar]
  6. Kennedy J. C., Till J. E., Siminovitch L., McCulloch E. A. The proliferative capacity of antigen-sensitive precursors of hemolytic plaque-forming cells. J Immunol. 1966 Jun;96(6):973–980. [PubMed] [Google Scholar]
  7. Playfair J. H., Papermaster B. W., Cole L. J. Focal antibody production by transferred spleen cells in irradiated mice. Science. 1965 Aug 27;149(3687):998–1000. doi: 10.1126/science.149.3687.998. [DOI] [PubMed] [Google Scholar]
  8. Simons M. J., Fowler R. Chorioallantoic membrane lesions produced by inoculation of adult fowl small lymphocytes. Nature. 1966 Feb 5;209(5023):588–589. doi: 10.1038/209588a0. [DOI] [PubMed] [Google Scholar]

Articles from Proceedings of the National Academy of Sciences of the United States of America are provided here courtesy of National Academy of Sciences

RESOURCES