Abstract
A macromolecular factor, TF1, has been isolated from bacteriophage SP01-infected B. subtilis. TF1 selectively inhibits in vitro transcription of SP01 and related viral DNA. Evidence is presented regarding these properties of the repressor-like TF1: (1) template and conformational specificity; (2) interaction with DNA rather than RNA polymerase; (3) trypsin sensitivity; (4) reversibility of action; and (5) ability to block initiation, but not propagation, of RNA synthesis.
Full text
PDF
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Bolle A., Epstein R. H., Salser W., Geiduschek E. P. Transcription during bacteriophage T4 development: synthesis and relative stability of early and late RNA. J Mol Biol. 1968 Feb 14;31(3):325–348. doi: 10.1016/0022-2836(68)90413-0. [DOI] [PubMed] [Google Scholar]
- CHAMBERLIN M., BERG P. Deoxyribo ucleic acid-directed synthesis of ribonucleic acid by an enzyme from Escherichia coli. Proc Natl Acad Sci U S A. 1962 Jan 15;48:81–94. doi: 10.1073/pnas.48.1.81. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Echols H., Pilarski L., Xcheng P. Y. In vitro repression of phage lambda DNA transcription by a partially purified repressor from lysogenic cells. Proc Natl Acad Sci U S A. 1968 Mar;59(3):1016–1023. doi: 10.1073/pnas.59.3.1016. [DOI] [PMC free article] [PubMed] [Google Scholar]
- GREEN D. M. INFECTIVITY OF DNA ISOLATED FROM BACILLUS SUBTILIS BACTERIOPHAGE, SP82. J Mol Biol. 1964 Dec;10:438–451. doi: 10.1016/s0022-2836(64)80065-6. [DOI] [PubMed] [Google Scholar]
- Gage L. P., Geiduschek E. P. Repression of early messenger transcription in the development of a bacteriophage. J Mol Biol. 1967 Dec 14;30(2):435–440. [PubMed] [Google Scholar]
- Gilbert W., Müller-Hill B. The lac operator is DNA. Proc Natl Acad Sci U S A. 1967 Dec;58(6):2415–2421. doi: 10.1073/pnas.58.6.2415. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Imamoto F., Morikawa N., Sato K. On the transcription of the tryptophan operon in Escherichia coli. 3. Multicistronic messenger RNA and polarity for transcription. J Mol Biol. 1965 Aug;13(1):169–182. doi: 10.1016/s0022-2836(65)80087-0. [DOI] [PubMed] [Google Scholar]
- JACOB F., MONOD J. Genetic regulatory mechanisms in the synthesis of proteins. J Mol Biol. 1961 Jun;3:318–356. doi: 10.1016/s0022-2836(61)80072-7. [DOI] [PubMed] [Google Scholar]
- OKUBO S., STRAUSS B., STODOLSKY M. THE POSSIBLE ROLE OF RECOMBINATION IN THE INFECTION OF COMPETENT BACILLUS SUBTILIS BY BACTERIOPHAGE DEOXYRIBONUCLEIC ACID. Virology. 1964 Dec;24:552–562. doi: 10.1016/0042-6822(64)90207-7. [DOI] [PubMed] [Google Scholar]
- Ptashne M., Hopkins N. The operators controlled by the lambda phage repressor. Proc Natl Acad Sci U S A. 1968 Aug;60(4):1282–1287. doi: 10.1073/pnas.60.4.1282. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Pène J. J., Marmur J. Deoxyribonucleic acid replication and expression of early and late bacteriophage functions in Bacillus subtilis. J Virol. 1967 Feb;1(1):86–91. doi: 10.1128/jvi.1.1.86-91.1967. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Sippel A., Hartmann G. Mode of action of rafamycin on the RNA polymerase reaction. Biochim Biophys Acta. 1968 Mar 18;157(1):218–219. doi: 10.1016/0005-2787(68)90286-4. [DOI] [PubMed] [Google Scholar]
- Wehrli W., Knüsel F., Schmid K., Staehelin M. Interaction of rifamycin with bacterial RNA polymerase. Proc Natl Acad Sci U S A. 1968 Oct;61(2):667–673. doi: 10.1073/pnas.61.2.667. [DOI] [PMC free article] [PubMed] [Google Scholar]