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. 1962 Aug;84(2):206–216. doi: 10.1128/jb.84.2.206-216.1962

COMPARISON OF THE DEVELOPMENTAL CYCLES OF SOME MEMBERS OF THE GENUS NOCARDIA1

James N Adams a, Norvel M McClung a
PMCID: PMC277837  PMID: 13859341

Abstract

Adams, James N. (University of Georgia, Athens) and Norvel M. McClung. Comparison of the developmental cycles of some members of the genus Nocardia. J. Bacteriol. 84:206–216. 1962.—A staining technique specific for cell walls was used to determine the characteristic development of Nocardia rubra, N. erythropolis, N. asteroides, and N. canicruria comb. nov. When inoculated on a fresh medium, these organisms were observed to germinate, with the production of one or more germ tubes from coccoid, ovoid, and short bacillary cells. Upon continued incubation, the germ tubes elongated to form filaments from which branches could be formed. Two other mechanisms of branching were noted. One was a result of multiple germination of cells and the other a result of single or multiple germination of short chains of cells. Both of these mechanisms could be readily differentiated from true branching. Mycelial formation varied in extent among the species studied and was governed, in part, by the types of branching which an organism exhibited. After the phase of branching development has been completed, fragmentation of the filaments and branches occurred as a result of the separation of the corresponding filament fragments following the random deposition of septa along the lengths of the cells. As the culture age increased, the separating filament fragments became shorter until they ultimately reached sizes like those of cells found in the inoculum. The observed developmental cycles of the genus Nocardia were compared to those which have been described for the genera Mycobacterium and Streptomyces, and the taxonomic implications of the differences between these developmental cycles was discussed.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. ADAMS J. N., McCLUNG N. M. Morphological studies in the genus Nocardia, 5. Septation in Nocardia rubra and Jensenia canicruria. J Bacteriol. 1960 Aug;80:281–282. doi: 10.1128/jb.80.2.281-282.1960. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. ADAMS J. N., McCLUNG N. M. On the nature of cytoplasmic inclusions of Nocardia rubra. J Gen Microbiol. 1962 Jun;28:231–241. doi: 10.1099/00221287-28-2-231. [DOI] [PubMed] [Google Scholar]
  3. BISSET K. A., MOORE F. W. Jensenia, a new genus of the actinomycetales. J Gen Microbiol. 1950 May;4(2):280–280. doi: 10.1099/00221287-4-2-280. [DOI] [PubMed] [Google Scholar]
  4. BRADLEY S. G. Sporulation by some strains of nocardiae and streptomycetes. Appl Microbiol. 1959 Mar;7(2):89–93. doi: 10.1128/am.7.2.89-93.1959. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Clark J. B., Frady J. SECONDARY LIFE CYCLE OF NOCARDIA CORALLINA. J Bacteriol. 1957 Nov;74(5):698–698. doi: 10.1128/jb.74.5.698-698.1957. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. DAVIS G. H., FREER J. H. Studies upon an oral aerobic actinomycete. J Gen Microbiol. 1960 Aug;23:163–178. doi: 10.1099/00221287-23-1-163. [DOI] [PubMed] [Google Scholar]
  7. DAVIS G. H. The interpretation of certain morphological appearances in a Streptomyces sp. J Gen Microbiol. 1960 Jun;22:740–743. doi: 10.1099/00221287-22-3-740. [DOI] [PubMed] [Google Scholar]
  8. GORDON R. E., MIHM J. M. A comparative study of some strains received as nocardiae. J Bacteriol. 1957 Jan;73(1):15–27. doi: 10.1128/jb.73.1.15-27.1957. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. GORDON R. E., MIHM J. M. A comparison of four species of mycobacteria. J Gen Microbiol. 1959 Dec;21:736–748. doi: 10.1099/00221287-21-3-736. [DOI] [PubMed] [Google Scholar]
  10. GORDON R. E., MIHM J. M. Sporulation by two strains of Nocardia asteroides. J Bacteriol. 1958 Feb;75(2):239–240. doi: 10.1128/jb.75.2.239-240.1958. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. HOPWOOD D. A. Phase-contrast observations on Streptomyces coelicolor. J Gen Microbiol. 1960 Feb;22:295–302. doi: 10.1099/00221287-22-1-295. [DOI] [PubMed] [Google Scholar]
  12. MORRIS E. O. Observations on the life cycle of the Nocardia. J Hyg (Lond) 1951 Jun-Sep;49(2-3):175–180. doi: 10.1017/s0022172400044077. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. McCLUNG N. M. Morphological studies in the genus Nocardia. II. Cytological studies. J Bacteriol. 1950 Apr;59(4):589–602. doi: 10.1128/jb.59.4.589-602.1950. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. McCLUNG N. M. Morphological studies in the genus Nocardia. III. The morphology of young colonies. Ann N Y Acad Sci. 1954 Oct 29;60(1):168–181. doi: 10.1111/j.1749-6632.1954.tb40006.x. [DOI] [PubMed] [Google Scholar]
  15. WEBB R. B. A useful bacterial cell wall stain. J Bacteriol. 1954 Feb;67(2):252–253. doi: 10.1128/jb.67.2.252-253.1954. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. WEBB R. B., CLARK J. B., CHANCE H. L. A cytological study of Nocardia corallina and other Actinomycetes. J Bacteriol. 1954 Apr;67(4):498–500. doi: 10.1128/jb.67.4.498-502.1954. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. WEBB R. B., CLARK J. B. Cytogenetic study of Nocardia corallina. J Bacteriol. 1957 Jul;74(1):31–42. doi: 10.1002/path.1700740106. [DOI] [PMC free article] [PubMed] [Google Scholar]

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