Skip to main content
PMC home page
Journal of Bacteriology logoLink to Journal of Bacteriology
. 1962 Aug;84(2):326–330. doi: 10.1128/jb.84.2.326-330.1962

APPLICATION OF THE MEMBRANE FILTER FOR THE QUANTITATIVE STUDY OF TRANSFORMATIONS WITH PARTICULAR REFERENCE TO PHENOTYPIC EXPRESSION OF AN ERYTHROMYCIN-RESISTANCE MUTATION

V N Iyer a,1
PMCID: PMC277865  PMID: 16561955

Abstract

Iyer, V. N. (University of Rochester, Rochester, N.Y.). Application of the membrane filter for the quantitative study of transformations with particular reference to phenotypic expression of an erythromycin-resistance mutation. J. Bacteriol. 84:326–330. 1962.—A technique using membrane filters for quantitative studies of transformation of pneumococci is described and some possible applications discussed. This method has been found amenable to the study of the integration and phenotypic expression of an erythromycin-resistance mutation of pneumococci which is characterized by a low frequency of transformation and delayed expression. The expression of survival occurs prior to the expression of colony-forming ability in the presence of erythromycin.

Full text

PDF
327

Images in this article

328Image
on p.328

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. FOX M. S., HOTCHKISS R. D. Initiation of bacterial transformation. Nature. 1957 Jun 29;179(4574):1322–1325. doi: 10.1038/1791322a0. [DOI] [PubMed] [Google Scholar]
  2. FOX M. S. Phenotypic expression of a genetic property introduced by deoxyribonucleate. J Gen Physiol. 1959 Mar 20;42(4):737–748. doi: 10.1085/jgp.42.4.737. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. HOTCHKISS R. D., EVANS A. H. Analysis of the complex sulfonamide resistance locus of pneumococcus. Cold Spring Harb Symp Quant Biol. 1958;23:85–97. doi: 10.1101/sqb.1958.023.01.012. [DOI] [PubMed] [Google Scholar]
  4. Hotchkiss R. D., Marmur J. DOUBLE MARKER TRANSFORMATIONS AS EVIDENCE OF LINKED FACTORS IN DESOXYRIBONUCLEATE TRANSFORMING AGENTS. Proc Natl Acad Sci U S A. 1954 Feb;40(2):55–60. doi: 10.1073/pnas.40.2.55. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. LACKS S., HOTCHKISS R. D. A study of the genetic material determining an enzyme in Pneumococcus. Biochim Biophys Acta. 1960 Apr 22;39:508–518. doi: 10.1016/0006-3002(60)90205-5. [DOI] [PubMed] [Google Scholar]
  6. LERMAN L. S., TOLMACH L. J. Genetic transformation. II. The significance of damage to the DNA molecule. Biochim Biophys Acta. 1959 Jun;33(2):371–387. doi: 10.1016/0006-3002(59)90127-1. [DOI] [PubMed] [Google Scholar]
  7. Marmur J., Lane D. STRAND SEPARATION AND SPECIFIC RECOMBINATION IN DEOXYRIBONUCLEIC ACIDS: BIOLOGICAL STUDIES. Proc Natl Acad Sci U S A. 1960 Apr;46(4):453–461. doi: 10.1073/pnas.46.4.453. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. RAVIN A. W., IYER V. N. The genetic relationship and phenotypic expression of mutations endowing Pneumococcus with resistance to erythromycin. J Gen Microbiol. 1961 Oct;26:277–301. doi: 10.1099/00221287-26-2-277. [DOI] [PubMed] [Google Scholar]
  9. RAVIN A. W. Reciprocal capsular transformations of pneumococci. J Bacteriol. 1959 Mar;77(3):296–309. doi: 10.1128/jb.77.3.296-309.1959. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. ROGER M., HOTCHKISS R. D. Selective heat inactivation of pneumococcal transforming deoxyribonucleate. Proc Natl Acad Sci U S A. 1961 May 15;47:653–669. doi: 10.1073/pnas.47.5.653. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Journal of Bacteriology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES