Abstract
Watanabe, Y. (406th Medical General Laboratory, U.S. Army Medical Center, Japan) and O. Felsenfeld. Serological analysis of supernatant liquids of cultures of El Tor vibrios. J. Bacteriol. 85:31–36. 1963.—Supernatant liquids of cultures of El Tor vibrios were analyzed serologically by applying the Ouchterlony agar diffusion technique. There are at least three different water-soluble antigenic components: thermolabile and exotoxin-like, thermolabile-bacterial, and thermostable-bacterial. All rabbit sera containing antibody(s) against the thermolabile exotoxin-like antigen(s) neutralized the lethal and hemolytic activities of the supernatant liquids of the cultures. These antibodies behave differently when absorbed with bacterial cells. The antihemolytic activity was not absorbed, whereas the antilethal was partially absorbed. The possibility of the existence of a nonactive toxin(s), which can stimulate production of antibodies against the lethal and hemolytic principles in the rabbit, was indicated.
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Selected References
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- Boroff D. A., Macri B. P. STUDY ON TOXINS AND ANTIGENS OF SHIGELLA DYSENTERIAE II. : Active Protection of Rabbits with Whole Organisms and Fractions of Shigella dysenteriae. J Bacteriol. 1949 Sep;58(3):387–394. [PMC free article] [PubMed] [Google Scholar]
- Boroff D. A. STUDY ON TOXINS AND ANTIGENS OF SHIGELLA DYSENTERIAE I. : Toxicity and Antigenicity of Whole Organisms and Various Fractions of Shigella dysenteriae. J Bacteriol. 1949 Jun;57(6):617–632. [PMC free article] [PubMed] [Google Scholar]
- JENKIN C. R., ROWLEY D. Toxic proteins from Vibrio cholerae and water vibrios which are lethal for mice. J Gen Microbiol. 1959 Aug;21:191–202. doi: 10.1099/00221287-21-1-191. [DOI] [PubMed] [Google Scholar]
- Morgan W. T., Partridge S. M. Studies in immunochemistry: The use of phenol and of alkali in the degradation of antigenic material isolated from Bact. dysenteriae (Shiga). Biochem J. 1941 Nov;35(10-11):1140–1163. doi: 10.1042/bj0351140. [DOI] [PMC free article] [PubMed] [Google Scholar]
- OUCHTERLONY O. Antigen-antibody reactions in gels. IV. Types of reactions in coordinated systems of diffusion. Acta Pathol Microbiol Scand. 1953;32(2):230–240. [PubMed] [Google Scholar]
- WATANABE Y., FELSENFELD O. Production of vibrio El Tor toxin in a liquid medium. J Bacteriol. 1961 Jul;82:43–47. doi: 10.1128/jb.82.1.43-47.1961. [DOI] [PMC free article] [PubMed] [Google Scholar]