Skip to main content
PMC home page
Journal of Bacteriology logoLink to Journal of Bacteriology
. 1963 Apr;85(4):808–815. doi: 10.1128/jb.85.4.808-815.1963

ISOLATION, ENUMERATION, AND CHARACTERISTICS OF PROTEOLYTIC RUMINAL BACTERIA1

Robert S Fulghum a,2, W E C Moore a
PMCID: PMC278229  PMID: 14044947

Abstract

Fulghum, Robert S. (Virginia Polytechnic Institute, Blacksburg) and W. E. C. Moore. Isolation, enumeration, and characteristics of proteolytic ruminal bacteria. J. Bacteriol. 85:808–815. 1963.—Colony counts of proteolytic ruminal bacteria in the order of 109 organisms per g of whole rumen contents, and total colony counts in the order of 2 to 3 × 109 organisms per g, were obtained from rumen contents of cattle fed a maintenance ration of hay and grain. The proteolytic counts averaged 38% of the total counts. An anaerobic, differential medium characterizing proteolytic colonies by clear zones in an opaque skim-milk suspension was utilized. Proteolytic isolates were assigned to the following taxa: Butyrivibrio sp., Succinivibrio sp., Selenomonas ruminantium var. lactilytica, Borrelia sp., Bacteroides sp., and selenomonadlike organisms similar to the B-385 group of Bryant.

Full text

PDF
809

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. APPLEBY J. C. The isolation and classification of proteolytic bacteria from the rumen of the sheep. J Gen Microbiol. 1955 Jun;12(3):526–533. doi: 10.1099/00221287-12-3-526. [DOI] [PubMed] [Google Scholar]
  2. BLACKBURN T. H., HOBSON P. N. Further studies on the isolation of proteolytic bacteria from the sheep rumen. J Gen Microbiol. 1962 Sep;29:69–81. doi: 10.1099/00221287-29-1-69. [DOI] [PubMed] [Google Scholar]
  3. BLACKBURN T. H., HOBSON P. N. Isolation of proteolytic bacteria from the sheep rumen. J Gen Microbiol. 1960 Feb;22:282–289. doi: 10.1099/00221287-22-1-282. [DOI] [PubMed] [Google Scholar]
  4. BLACKBURN T. H., HOBSON P. N. Proteolysis in the sheep rumen by whole and fractionated rumen contents. J Gen Microbiol. 1960 Feb;22:272–281. doi: 10.1099/00221287-22-1-272. [DOI] [PubMed] [Google Scholar]
  5. BRYANT M. P. Bacterial species of the rumen. Bacteriol Rev. 1959 Sep;23(3):125–153. doi: 10.1128/br.23.3.125-153.1959. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. BRYANT M. P., SMALL N., BOUMA C., CHU H. Bacteroides ruminicola n. sp. and Succinimonas amylolytica; the new genus and species; species of succinic acid-producing anaerobic bacteria of the bovine rumen. J Bacteriol. 1958 Jul;76(1):15–23. doi: 10.1128/jb.76.1.15-23.1958. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. BRYANT M. P., SMALL N. Characteristics of two new genera of anaerobic curved rods isolated from the rumen of cattle. J Bacteriol. 1956 Jul;72(1):22–26. doi: 10.1128/jb.72.1.22-26.1956. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. BRYANT M. P., SMALL N. The anaerobic monotrichous butyric acid-producing curved rod-shaped bacteria of the rumen. J Bacteriol. 1956 Jul;72(1):16–21. doi: 10.1128/jb.72.1.16-21.1956. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. BRYANT M. P. The characteristics of strains of Selenomonas isolated from bovine rumen contents. J Bacteriol. 1956 Aug;72(2):162–167. doi: 10.1128/jb.72.2.162-167.1956. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. BRYANT M. P. The isolation and characteristics of a spirochete from the bovine rumen. J Bacteriol. 1952 Sep;64(3):325–335. doi: 10.1128/jb.64.3.325-335.1952. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. HAMLIN L. J., HUNGATE R. E. Culture and physiology of a starch-digesting bacterium (Bacteroides amylophilus n. sp.) from the bovine rumen. J Bacteriol. 1956 Oct;72(4):548–554. doi: 10.1128/jb.72.4.548-554.1956. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. HUHTANEN C. N., GALL L. S. Rumen organisms. II. Two lactate utilizers and six miscellaneous types. J Bacteriol. 1953 May;65(5):554–559. doi: 10.1128/jb.65.5.554-559.1953. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. HUNGATE R. E. Microorganisms in the rumen of cattle fed a constant ration. Can J Microbiol. 1957 Mar;3(2):289–311. doi: 10.1139/m57-034. [DOI] [PubMed] [Google Scholar]
  14. HUNGATE R. E. The anaerobic mesophilic cellulolytic bacteria. Bacteriol Rev. 1950 Mar;14(1):1–49. doi: 10.1128/br.14.1.1-49.1950. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. KING K. W., SMITH P. H. Comparisons of two media proposed for the isolation of bacteria from the rumen. J Bacteriol. 1955 Dec;70(6):726–729. doi: 10.1128/jb.70.6.726-729.1955. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. MANN S. O., MASSON F. M., OXFORD A. E. Facultative anaerobic bacteria from the sheep's rumen. J Gen Microbiol. 1954 Feb;10(1):142–149. doi: 10.1099/00221287-10-1-142. [DOI] [PubMed] [Google Scholar]
  17. VELDKAMP H. Isolation and characteristics of Treponema zuelzerae nov. spec., and anaerobic, free-living spirochete. Antonie Van Leeuwenhoek. 1960;26:103–125. doi: 10.1007/BF02538999. [DOI] [PubMed] [Google Scholar]

Articles from Journal of Bacteriology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES