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. 1963 Jun;85(6):1261–1273. doi: 10.1128/jb.85.6.1261-1273.1963

SEROLOGICAL RELATIONSHIPS AMONG HUMAN MYCOPLASMAS AS SHOWN BY COMPLEMENT-FIXATION AND GEL DIFFUSION

David Taylor-Robinson a,1, Norman L Somerson a, Horace C Turner a, Robert M Chanock a
PMCID: PMC278327  PMID: 14047216

Abstract

Taylor-Robinson, David (National Institutes of Health, Bethesda, Md.), Norman L. Somerson, Horace C. Turner, and Robert M. Chanock. Serological relationships among human mycoplasmas as shown by complement-fixation and gel diffusion. J. Bacteriol. 85:1261–1273. 1963.—Antigenic relationships among human mycoplasmas were studied by complement-fixation and agar gel diffusion techniques. Four recognized human species Mycoplasma hominis type 1, M. hominis type 2, M. salivarium, and M. fermentans were antigenically distinct in these tests. In addition, M. pneumoniae (Eaton agent, the etiological agent of cold agglutinin-positive atypical pneumonia) was different from these four species. Although these species were distinct, evidence of shared antigenic components was obtained in complement-fixation and agar gel diffusion tests. Since rabbits were immunized with mycoplasmas grown in rabbit muscle infusion broth supplemented with rabbit serum or, in the case of M. pneumoniae, with infected chick embryo lung suspension, the possibility that the heterologous reactions resulted from antibody to growth medium components could be excluded. Four recent mycoplasma isolates from the oropharynx were analyzed, and three were shown to be closely related to M. hominis type 1, and the fourth was closely related to M. salivarium. Although the recent isolates could not be distinguished from the related “prototype” human species by complement-fixation, differences could be detected by the agar gel diffusion technique.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. CARD D. H. PPLO of human genital origin; serological classification of strains and antibody distribution in man. Br J Vener Dis. 1959 Mar;35(1):27–34. doi: 10.1136/sti.35.1.27. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. CHANOCK R. M., HAYFLICK L., BARILE M. F. Growth on artificial medium of an agent associated with atypical pneumonia and its identification as a PPLO. Proc Natl Acad Sci U S A. 1962 Jan 15;48:41–49. doi: 10.1073/pnas.48.1.41. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. CHANOCK R. M., JAMES W. D., FOX H. H., TURNER H. C., MUFSON M. A., HAYFLICK L. Growth of Eaton PPLO in broth and preparation of complement fixing antigen. Proc Soc Exp Biol Med. 1962 Aug-Sep;110:884–889. doi: 10.3181/00379727-110-27681. [DOI] [PubMed] [Google Scholar]
  4. CLARK H. W., FOWLER R. C., BROWN T. M. Preparation of pleuropneumonia-like organisms for microscopic study. J Bacteriol. 1961 Mar;81:500–502. doi: 10.1128/jb.81.3.500-502.1961. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. CORIELL L. L., FABRIZIO D. P., WILSON S. R. Comparison of PPLO strains from tissue culture by complement fixation. Ann N Y Acad Sci. 1960 Jan 15;79:574–580. doi: 10.1111/j.1749-6632.1960.tb42726.x. [DOI] [PubMed] [Google Scholar]
  6. EDWARD D. G., FREUNDT E. A. The classification and nomenclature of organisms of the pleuropneumonia group. J Gen Microbiol. 1956 Feb;14(1):197–207. doi: 10.1099/00221287-14-1-197. [DOI] [PubMed] [Google Scholar]
  7. LIU C. Studies on primary atypical pneumonia. I. Localization, isolation, and cultivation of a virus in chick embryos. J Exp Med. 1957 Oct 1;106(4):455–466. doi: 10.1084/jem.106.4.455. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. NICOL C. S., EDWARD D. G. Role of organisms of the pleuropneumonia group in human genital infections. Br J Vener Dis. 1953 Sep;29(3):141–150. doi: 10.1136/sti.29.3.141. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. RUITER M., WENTHOLT H. M. Isolation of a pleuropneumonia-like organism (G-strain) in a case of fusospirillary vulvovaginitis. Acta Derm Venereol. 1953;33(1-2):123–129. [PubMed] [Google Scholar]
  10. SEVER J. L. Application of a microtechnique to viral serological investigations. J Immunol. 1962 Mar;88:320–329. [PubMed] [Google Scholar]
  11. SOMERSON N. L., TAYLOR-ROBINSON D., CHANOCK R. M. Hemolyin production as an aid in the identification and quantitation of Eaton agent (Mycoplasma pneumoniae). Am J Hyg. 1963 Jan;77:122–128. doi: 10.1093/oxfordjournals.aje.a120290. [DOI] [PubMed] [Google Scholar]
  12. URIEL J., GRABAR P. Emploi de colorants dans l'analyse électrophorétique et immuno électrophorétique en milieu gélifié. Ann Inst Pasteur (Paris) 1956 Apr;90(4):427–440. [PubMed] [Google Scholar]

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