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. 1963 Oct;86(4):855–860. doi: 10.1128/jb.86.4.855-860.1963

SEROLOGICAL ANALYSIS OF BUTYRIVIBRIO FROM THE BOVINE RUMEN

S S Margherita a,1, R E Hungate a
PMCID: PMC278525  PMID: 14066485

Abstract

Margherita, S. S. (University of California, Davis) and R. E. Hungate. Serological analysis of Butyrivibrio from the bovine rumen. J. Bacteriol. 86:855–860. 1963.—The cultural and fermentation characteristics of a number of strains of Butyrivibrio fibrisolvens isolated from the bovine rumen of cattle from different areas were determined, and the strains were subjected to serological analysis by the techniques of agglutination, immunodiffusion, and indirect hemolysis. In general, the results of the three methods agreed fairly well, but some variation was shown according to the method of preparation of the antigen. Much serological heterogeneity was disclosed. The greatest degree of agglutinating cross-reactivity was observed with strains isolated simultaneously from two animals in the same herd of African zebu cattle and with a Pullman strain. These cross-reactions were confirmed by immunodiffusion and indirect hemolysis tests. Agglutinating cross-reactions at low titers were observed between additional strains. The African isolates were shown to possess unique, as well as shared, antigens.

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Selected References

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  1. BALAZS V. Studies on the antibody content of antacid gastric juices. Experientia. 1962 Feb 15;18:72–73. doi: 10.1007/BF02138263. [DOI] [PubMed] [Google Scholar]
  2. BRYANT M. P., SMALL N. The anaerobic monotrichous butyric acid-producing curved rod-shaped bacteria of the rumen. J Bacteriol. 1956 Jul;72(1):16–21. doi: 10.1128/jb.72.1.16-21.1956. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. HOBSON P. N., MANN S. O. Some studies on the identification of rumen bacteria with fluorescent antibodies. J Gen Microbiol. 1957 Apr;16(2):463–471. doi: 10.1099/00221287-16-2-463. [DOI] [PubMed] [Google Scholar]
  4. HUNGATE R. E. The anaerobic mesophilic cellulolytic bacteria. Bacteriol Rev. 1950 Mar;14(1):1–49. doi: 10.1128/br.14.1.1-49.1950. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. MORTON R. K. Separation and purification of enzymes associated with insoluble particles. Nature. 1950 Dec 30;166(4235):1092–1095. doi: 10.1038/1661092a0. [DOI] [PubMed] [Google Scholar]
  6. Palmer J. W. A SIMPLE METHOD FOR PREPARING ANTIGENIC SUBSTANCES FROM THE TYPHOID BACILLUS. Science. 1940 Aug 16;92(2381):155–156. doi: 10.1126/science.92.2381.155. [DOI] [PubMed] [Google Scholar]

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