Abstract
Rabin, Robert (Albert Einstein Medical Center, Philadelphia, Pa.), Henry C. Reeves, and Samuel J. Ajl. β-Ethylmalate synthetase. J. Bacteriol. 86:937–944. 1963.—Pseudomonas aeruginosa, when grown in an inorganic salts medium with butyrate as the sole carbon source, produced an enzyme which catalyzed the condensation of butyryl coenzyme A (CoA) and glyoxylate to form β-ethylmalic acid. Maximal activity was found in the 40 to 60% (NH4)2SO4 fraction of cell-free extracts. Mg++ was stimulatory, and the optimal pH was 8.0 to 8.5. β-Ethylmalic acid-C14 was demonstrable in paper chromatograms when either glyoxylate-1-C14 or butyryl CoA-3-C14 was included in complete systems incubated anaerobically. When either glyoxylate, butyryl CoA, or enzyme was omitted, β-ethylmalate was not detected. Further evidence that β-ethylmalic acid-C14 was the product of the condensation of butyryl CoA-3-C14 and nonlabeled glyoxylate was provided by synthesis of the derivative 5-ethyluracil-C14. The derivative was recrystallized to constant specific activity and identified by its melting point and spectral properties.
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Selected References
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- BURCKHALTER J. H., SCARBOROUGH H. C. The synthesis of uracils as anticonvulsants. J Am Pharm Assoc Am Pharm Assoc. 1955 Sep;44(9):545–550. doi: 10.1002/jps.3030440910. [DOI] [PubMed] [Google Scholar]
- GUYOT A., MENTZER C. Sur une nouvelle synthèse de la thymine. C R Hebd Seances Acad Sci. 1958 Jan 20;246(3):436–438. [PubMed] [Google Scholar]
- KORNBERG H. L., ELSDEN S. R. The metabolism of 2-carbon compounds by microorganisms. Adv Enzymol Relat Subj Biochem. 1961;23:401–470. doi: 10.1002/9780470122686.ch8. [DOI] [PubMed] [Google Scholar]
- REEVES H. C., AJL S. J. Alpha-hydroxyglutaric acid synthetase. J Bacteriol. 1962 Jul;84:186–187. doi: 10.1128/jb.84.1.186-187.1962. [DOI] [PMC free article] [PubMed] [Google Scholar]
- REEVES H. C., AJL S. J. Enzymatic formation of lactate and acetate from alpha-hydroxyglutarate. Biochem Biophys Res Commun. 1963 Jul 18;12:132–136. doi: 10.1016/0006-291x(63)90248-1. [DOI] [PubMed] [Google Scholar]
- REEVES H. C., AJL S. Occurrence and function of isocitritase and malate synthetase in bacteria. J Bacteriol. 1960 Mar;79:341–345. doi: 10.1128/jb.79.3.341-345.1960. [DOI] [PMC free article] [PubMed] [Google Scholar]
- WONG D. T., AJL S. J. Significance of the malate synthetase reaction in bacteria. Science. 1957 Nov 15;126(3281):1013–1014. doi: 10.1126/science.126.3281.1013. [DOI] [PubMed] [Google Scholar]