Abstract
Law, John H. (Harvard University, Cambridge, Mass.) and Ralph A. Splepecky. Assay of poly-β-hydroxybutyric acid. J. Bacteriol. 82:33–36. 1961—A convenient spectrophotometric assay of bacterial poly-β-hydroxybutyric acid has been devised. Quantitative conversion of poly-β-hydroxybutyric acid to crotonic acid by heating in concentrated sulfuric acid and determination of the ultraviolet absorption of the produce permits an accurate determination of this material in quantities down to 5 μg. This method has been used to follow the production of poly-β-hydroxybutyric acid by Bacillus megaterium strain KM.
Full text
PDF
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- DOUDOROFF M., STANIER R. Y. Role of poly-beta-hydroxybutyric acid in the assimilation of organic carbon by bacteria. Nature. 1959 May 23;183(4673):1440–1442. doi: 10.1038/1831440a0. [DOI] [PubMed] [Google Scholar]
- FORSYTH W. G., HAYWARD A. C., ROBERTS J. B. Occurrence of poly-beta-hydroxybutyric acid in aerobic gram-negative bacteria. Nature. 1958 Sep 20;182(4638):800–801. doi: 10.1038/182800a0. [DOI] [PubMed] [Google Scholar]
- HAYWARD A. C., FORSYTH W. G., ROBERTS J. B. Synthesis and breakdown of poly-beta-hydroxybutyric acid by bacteria. J Gen Microbiol. 1959 Jun;20(3):510–518. doi: 10.1099/00221287-20-3-510. [DOI] [PubMed] [Google Scholar]
- KALLIO R. E., HARRINGTON A. A. Sudanophilic granules and lipid of Pseudomonas methanica. J Bacteriol. 1960 Sep;80:321–324. doi: 10.1128/jb.80.3.321-324.1960. [DOI] [PMC free article] [PubMed] [Google Scholar]
- MACRAE R. M., WILKINSON J. F. Poly-beta-hyroxybutyrate metabolism in washed suspensions of Bacillus cereus and Bacillus megaterium. J Gen Microbiol. 1958 Aug;19(1):210–222. doi: 10.1099/00221287-19-1-210. [DOI] [PubMed] [Google Scholar]
- MORRIS M. B., ROBERTS J. B. A group of pseudomonads able to synthesize poly-beta-hydroxybutyric acid. Nature. 1959 May 30;183(4674):1538–1539. doi: 10.1038/1831538a0. [DOI] [PubMed] [Google Scholar]
- Stanier R. Y., Doudoroff M., Kunisawa R., Contopoulou R. THE ROLE OF ORGANIC SUBSTRATES IN BACTERIAL PHOTOSYNTHESIS. Proc Natl Acad Sci U S A. 1959 Aug;45(8):1246–1260. doi: 10.1073/pnas.45.8.1246. [DOI] [PMC free article] [PubMed] [Google Scholar]
- WEIBULL C. Characterization of the protoplasmic constituents of bacillus megaterium. J Bacteriol. 1953 Dec;66(6):696–702. doi: 10.1128/jb.66.6.696-702.1953. [DOI] [PMC free article] [PubMed] [Google Scholar]
- WILLIAMSON D. H., WILKINSON J. F. The isolation and estimation of the poly-beta-hydroxybutyrate inclusions of Bacillus species. J Gen Microbiol. 1958 Aug;19(1):198–209. doi: 10.1099/00221287-19-1-198. [DOI] [PubMed] [Google Scholar]