Abstract
Lewis, Vester J., Jr. (University of Oklahoma School of Medicine, Oklahoma City) and L. Vernon Scott. Nutritional requirements for the production of herpes simplex virus. I. Influence of glucose and glutamine on herpes simplex virus production by HeLa cells. J. Bacteriol. 83:475–482. 1962.—The importance of glucose and glutamine in herpes simplex virus production by HeLa cells was evaluated by variation of the composition of Eagle's basal medium. Simultaneous omission of glucose and glutamine from the serum-containing medium resulted in marked curtailment of viral synthesis. The effect was attributable neither to decreased survival time of infectious particles or of cells, nor to decreased rate of viral adsorption or penetration in the presence of the deficient medium. Therefore, the effect was probably on intracellular viral replication.
When the requirements for glucose and glutamine were determined separately, it was found that viral production was reduced in glucose-deficient medium. In contrast, a medium which was complete except for restriction of the glutamine content to that occurring naturally in the serum component supported significantly greater viral multiplication than did the complete medium. Limited viral replication occurred with medium which lacked both glutamine and serum. Viral multiplication in the presence of serum-free medium was stimulated by the addition of glutamine.
Full text
PDF
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- DARNELL J. E., Jr, EAGLE H. Glucose and glutamine in poliovirus production by HeLa cells. Virology. 1958 Oct;6(2):556–566. doi: 10.1016/0042-6822(58)90102-8. [DOI] [PubMed] [Google Scholar]
- EAGLE H., HABEL K. The nutritional requirements for the propagation of poliomyelitis virus by the HeLa cell. J Exp Med. 1956 Aug 1;104(2):271–287. doi: 10.1084/jem.104.2.271. [DOI] [PMC free article] [PubMed] [Google Scholar]
- EAGLE H. The specific amino acid requirements of a human carcinoma cell (Stain HeLa) in tissue culture. J Exp Med. 1955 Jul 1;102(1):37–48. doi: 10.1084/jem.102.1.37. [DOI] [PMC free article] [PubMed] [Google Scholar]
- EATON M. D., MAGASANIK B., PERRY M. E., KARIBIAN D. Inhibition of influenza and mumps viruses in tissue culture by basic amino acids. Proc Soc Exp Biol Med. 1951 Jul;77(3):505–508. doi: 10.3181/00379727-77-18829. [DOI] [PubMed] [Google Scholar]
- FARNHAM A. E. The formation of microscopic plaques by herpes simplex virus in HeLa cells. Virology. 1958 Oct;6(2):317–327. doi: 10.1016/0042-6822(58)90085-0. [DOI] [PubMed] [Google Scholar]
- TYNDALL R. L., LUDWIG E. H. Nutritional requirements for the production of poliovirus type II. Coxsackie B3, and vaccinia viruses by continuous animal cell cultures. J Bacteriol. 1960 Jul;80:96–103. doi: 10.1128/jb.80.1.96-103.1960. [DOI] [PMC free article] [PubMed] [Google Scholar]