Abstract
Madoff, Morton A. (New England Center Hospital, Boston, Mass.) and Louis Weinstein. Purification of staphylococcal alpha-hemolysin. J. Bacteriol. 83:914–918. 1962. — Staphylococcal alpha-toxin has been purified by metal-ion precipitation, column chromatography, and continuous-flow paper electrophoresis. The resultant preparation is highly unstable. A single line is obtained on agar-gel diffusion analysis against staphylococcal antiserum. Two bands, both associated with hemolytic activity and migrating cathodally at pH 8.4, are obtained on starch-gel electrophoresis. Levels of lytic, dermonecrotic, and lethal toxin activity maintain a constant relationship in the crude and purified materials, suggesting the unity of these effects.
Full text
PDF
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- BLOBEL H., BERMAN D. T., SIMON J. Purification of staphylococcal coagulase. J Bacteriol. 1960 Jun;79:807–815. doi: 10.1128/jb.79.6.807-815.1960. [DOI] [PMC free article] [PubMed] [Google Scholar]
- BUTLER L. O. Studies on the preparation and isoelectric point of staphylococcal alpha-haemolysin. Biochem J. 1959 Jan;71(1):67–73. doi: 10.1042/bj0710067. [DOI] [PMC free article] [PubMed] [Google Scholar]
- FERGUSON K. A., WALLACE A. L. Starch-gel electrophoresis of anterior pituitary hormones. Nature. 1961 May 13;190:629–630. doi: 10.1038/190629a0. [DOI] [PubMed] [Google Scholar]
- HAUSLER W. J., Jr, DICK E. C. The purification and concentration of influenza A virus (PR8) by a method of continuous extraction with glycine of a zinc-virus precipitate. J Infect Dis. 1960 Sep-Oct;107:189–194. doi: 10.1093/infdis/107.2.189. [DOI] [PubMed] [Google Scholar]
- LACK C. H., WAILLING D. G. A study of 435 strains of Staphylococcus pyogenes with reference to factors which may contribute to pathogenicity. J Pathol Bacteriol. 1954 Oct;68(2):431–443. doi: 10.1002/path.1700680217. [DOI] [PubMed] [Google Scholar]
- LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
- MADOFF M. A., EYLAR E. H., WEINSTEIN L. Serologic studies of the neuraminidases of Vibrio cholerae, Diplococcus pneumoniae and influenza virus. J Immunol. 1960 Dec;85:603–613. [PubMed] [Google Scholar]
- ROBINSON J., THATCHER F. S., MONTFORD J. Studies with staphylococcal toxins. V. Possible identification of alpha hemolysin with a proteolytic enzyme. Can J Microbiol. 1960 Apr;6:183–194. doi: 10.1139/m60-020. [DOI] [PubMed] [Google Scholar]
- SPIVACK M. L., KARLER A. Purification of the toxin of Pasteurella pestis by continuous-flow paper electrophoresis. J Immunol. 1958 Jun;80(6):441–445. [PubMed] [Google Scholar]
- TURPIN A., RELYVELD E. H., PILLET J., RAYNAUD M. Purification de la toxine et de l'anatoxine staphylococciques alpha. Ann Inst Pasteur (Paris) 1954 Aug;87(2):185–193. [PubMed] [Google Scholar]