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. 1988 Mar;85(6):1826–1830. doi: 10.1073/pnas.85.6.1826

DNA bending is induced in an enhancer by the DNA-binding domain of the bovine papillomavirus E2 protein.

C Moskaluk 1, D Bastia 1
PMCID: PMC279872  PMID: 2831538

Abstract

The E2 gene of bovine papillomavirus type 1 has been shown to encode a DNA-binding protein and to trans-activate the viral enhancer. We have localized the DNA-binding domain of the E2 protein to the carboxyl-terminal 126 amino acids of the E2 open reading frame. The DNA-binding domain has been expressed in Escherichia coli and partially purified. Gel retardation and DNase I "footprinting" on the bovine papillomavirus type 1 enhancer identify the sequence motif ACCN6GGT (in which N = any nucleotide) as the E2 binding site. Using electrophoretic methods we have shown that the DNA-binding domain changes conformation of the enhancer by inducing significant DNA bending.

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