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. 1993 May;61(5):2048–2052. doi: 10.1128/iai.61.5.2048-2052.1993

Protective immunity induced in Aotus monkeys by a recombinant SERA protein of Plasmodium falciparum: further studies using SERA 1 and MF75.2 adjuvant.

J Inselburg 1, I C Bathurst 1, J Kansopon 1, P J Barr 1, R Rossan 1
PMCID: PMC280802  PMID: 8478093

Abstract

We describe the third of three vaccination trials of Panamanian Aotus monkeys with a recombinant blood-stage antigen derived from the malaria parasite Plasmodium falciparum. Immunization was performed with an N-terminal region of the SERA antigen (serine repeat antigen protein), SERA 1, that contains a 262-amino-acid fragment including amino acids 24 to 285 of the 989-amino-acid SERA protein. Vaccinations were carried out with the recombinant protein mixed with either Freund's, MF75.2, or MF59.2 adjuvant. A control group that did not receive SERA 1 but only MF75.2 adjuvant was included. Monkeys vaccinated with the antigen MF59.2 mixture produced low anti-SERA 1 titers and were not protected. Monkeys vaccinated with antigen and Freund's adjuvant had, in general, a higher average anti-SERA 1 titer (107,278) than did monkeys immunized with SERA 1 and MF75.2 (40, 143), yet monkeys in both groups were well protected. Monkeys that received only MF75.2 developed neither detectable anti-SERA 1 nor anti-P. falciparum antibodies prior to or 10 days after parasite challenge, yet were apparently protected against infection. Monkeys vaccinated with either SERA 1 and Freund's, SERA 1 and MF75.2, or MF75.2 alone and that had been challenged but did not develop a countable parasitemia were treated with a curative dose of mefloquine 100 days after parasite challenge and then rechallenged 40 days later. None of the five rechallenged monkeys that had originally received SERA 1 and Freund's developed a countable parasitemia. Only one of five rechallenged monkeys that originally received SERA 1 and MF75.2 developed a high countable parasitemia, while two animals developed a barely countable parasitemia. Four of the rechallenged monkeys that had originally received only MF75.2 developed a moderate to high countable parasitemia. The results indicate that vaccination with SERA 1 and either Freund's or MF75.2 adjuvant provides protection and vaccination with MF75.2 alone can provide a temporary protection unrelated to the induction of anti-SERA 1 or antimalarial antibodies.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Banyal H. S., Inselburg J. Isolation and characterization of parasite-inhibitory Plasmodium falciparum monoclonal antibodies. Am J Trop Med Hyg. 1985 Nov;34(6):1055–1064. doi: 10.4269/ajtmh.1985.34.1055. [DOI] [PubMed] [Google Scholar]
  2. Barr P. J., Inselburg J., Green K. M., Kansopon J., Hahm B. K., Gibson H. L., Lee-Ng C. T., Bzik D. J., Li W. B., Bathurst I. C. Immunogenicity of recombinant Plasmodium falciparum SERA proteins in rodents. Mol Biochem Parasitol. 1991 Mar;45(1):159–170. doi: 10.1016/0166-6851(91)90038-8. [DOI] [PubMed] [Google Scholar]
  3. Bzik D. J., Li W. B., Horii T., Inselburg J. Amino acid sequence of the serine-repeat antigen (SERA) of Plasmodium falciparum determined from cloned cDNA. Mol Biochem Parasitol. 1988 Sep;30(3):279–288. doi: 10.1016/0166-6851(88)90097-7. [DOI] [PubMed] [Google Scholar]
  4. Engvall E., Perlmann P. Enzyme-linked immunosorbent assay, Elisa. 3. Quantitation of specific antibodies by enzyme-labeled anti-immunoglobulin in antigen-coated tubes. J Immunol. 1972 Jul;109(1):129–135. [PubMed] [Google Scholar]
  5. Horii T., Bzik D. J., Inselburg J. Characterization of antigen-expressing Plasmodium falciparum cDNA clones that are reactive with parasite inhibitory antibodies. Mol Biochem Parasitol. 1988 Jul;30(1):9–18. doi: 10.1016/0166-6851(88)90127-2. [DOI] [PubMed] [Google Scholar]
  6. Inselburg J., Bathurst I. C., Kansopon J., Barchfeld G. L., Barr P. J., Rossan R. N. Protective immunity induced in Aotus monkeys by a recombinant SERA protein of Plasmodium falciparum: adjuvant effects on induction of protective immunity. Infect Immun. 1993 May;61(5):2041–2047. doi: 10.1128/iai.61.5.2041-2047.1993. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Inselburg J., Bzik D. J., Li W. B., Green K. M., Kansopon J., Hahm B. K., Bathurst I. C., Barr P. J., Rossan R. N. Protective immunity induced in Aotus monkeys by recombinant SERA proteins of Plasmodium falciparum. Infect Immun. 1991 Apr;59(4):1247–1250. doi: 10.1128/iai.59.4.1247-1250.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Inselburg J. Gametocyte formation by the progeny of single Plasmodium falciparum schizonts. J Parasitol. 1983 Jun;69(3):584–591. [PubMed] [Google Scholar]
  9. Inselburg J., Rossan R. N., Escajadillo A. Growth and immunity conferred by a Plasmodium falciparum temperature sensitive mutant in Panamanian owl monkeys. Am J Trop Med Hyg. 1989 May;40(5):465–469. doi: 10.4269/ajtmh.1989.40.465. [DOI] [PubMed] [Google Scholar]
  10. Knapp B., Hundt E., Nau U., Küpper H. A. Molecular cloning, genomic structure and localization in a blood stage antigen of Plasmodium falciparum characterized by a serine stretch. Mol Biochem Parasitol. 1989 Jan 1;32(1):73–83. doi: 10.1016/0166-6851(89)90131-x. [DOI] [PubMed] [Google Scholar]
  11. Knapp B., Nau U., Hundt E., Küpper H. A. A new blood stage antigen of Plasmodium falciparum highly homologous to the serine-stretch protein SERP. Mol Biochem Parasitol. 1991 Jan;44(1):1–13. doi: 10.1016/0166-6851(91)90215-r. [DOI] [PubMed] [Google Scholar]
  12. Rossan R. N., Harper J. S., 3rd, Davidson D. E., Jr, Escajadillo A., Christensen H. A. Comparison of Plasmodium falciparum infections in Panamanian and Colombian owl monkeys. Am J Trop Med Hyg. 1985 Nov;34(6):1037–1047. doi: 10.4269/ajtmh.1985.34.1037. [DOI] [PubMed] [Google Scholar]

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