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. 1993 Jun;61(6):2400–2407. doi: 10.1128/iai.61.6.2400-2407.1993

Immunoglobulin class and subclass distribution of antibodies reactive with the immunodominant antigen of Actinobacillus actinomycetemcomitans serotype b.

H Lu 1, J V Califano 1, H A Schenkein 1, J G Tew 1
PMCID: PMC280862  PMID: 8500879

Abstract

The aims of this study were to determine the immunodominant antigens of Actinobacillus actinomycetemcomitans serotype b (Aab) for the different immunoglobulin (Ig) classes and subclasses and to determine the relative levels of these different Igs in serum. Seropositive early-onset periodontitis patients were sampled, and the Ig classes IgG, IgA, and IgM and subclasses IgG1, IgG2, IgG3, IgG4, IgA1, and IgA2 were studied. Reactivity with Aab antigens was assessed by using the Western blot (immunoblot) in limiting dilution analysis and radioimmunoassay with sera from 13 early-onset periodontitis subjects. A smeared antigen in the upper portion of the immunoblots, typical of high-molecular-weight LPS, was immunodominant for IgG, IgA, IgM, IgG1, IgG2, IgG3, IgA1, and IgA2. This smeared antigen was present in every patient for all of these Igs at the endpoint. A few additional antigens were also present at the endpoint in some patients, but none were present in more than half of the subjects. The distribution of antibody titers by Ig classes reactive with the Aab immunodominant antigen was IgG > IgA > IgM. The distribution of antibody titers by IgG subclass was IgG2 > IgG1 approximately IgG3. Further quantitation by radioimmunoassay revealed that the mean concentration of IgG2 (65.7 micrograms/ml) was significantly greater than that of IgG1 (8.8 micrograms/ml). The IgA subclass distribution was IgA1 >> IgA2, with IgA1 apparently being second only to IgG2. Therefore, the Aab antigen eliciting the highest antibody level in virtually all Ig classes and subclasses appeared to be lipopolysaccharide, and IgG2 was markedly elevated over all other serum Ig classes or subclasses reactive with Aab.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bonta Y., Zambon J. J., Genco R. J., Neiders M. E. Rapid identification of periodontal pathogens in subgingival plaque: comparison of indirect immunofluorescence microscopy with bacterial culture for detection of Actinobacillus actinomycetemcomitans. J Dent Res. 1985 May;64(5):793–798. doi: 10.1177/00220345850640050201. [DOI] [PubMed] [Google Scholar]
  2. Brown T. A., Byres L., Gardner M., Van Dyke T. E. Subclass and molecular form of immunoglobulin A antibodies to Actinobacillus actinomycetemcomitans in juvenile periodontitis. Infect Immun. 1991 Mar;59(3):1126–1130. doi: 10.1128/iai.59.3.1126-1130.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Califano J. V., Schenkein H. A., Tew J. G. Immunodominant antigen of Actinobacillus actinomycetemcomitans Y4 in high-responder patients. Infect Immun. 1989 May;57(5):1582–1589. doi: 10.1128/iai.57.5.1582-1589.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Califano J. V., Schenkein H. A., Tew J. G. Immunodominant antigens of Actinobacillus actinomycetemcomitans serotype b in early-onset periodontitis patients. Oral Microbiol Immunol. 1992 Apr;7(2):65–70. doi: 10.1111/j.1399-302x.1992.tb00511.x. [DOI] [PubMed] [Google Scholar]
  5. Ebersole J. L., Sandoval M. N., Steffen M. J., Cappelli D. Serum antibody in Actinobacillus actinomycetemcomitans-infected patients with periodontal disease. Infect Immun. 1991 May;59(5):1795–1802. doi: 10.1128/iai.59.5.1795-1802.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Ebersole J. L., Taubman M. A., Smith D. J., Genco R. J., Frey D. E. Human immune responses to oral micro-organisms. I. Association of localized juvenile periodontitis (LJP) with serum antibody responses to Actinobacillus actinomycetemcomitans. Clin Exp Immunol. 1982 Jan;47(1):43–52. [PMC free article] [PubMed] [Google Scholar]
  7. Gunsolley J. C., Tew J. G., Gooss C. M., Burmeister J. A., Schenkein H. A. Effects of race and periodontal status on antibody reactive with Actinobacillus actinomycetemcomitans strain Y4. J Periodontal Res. 1988 Sep;23(5):303–307. doi: 10.1111/j.1600-0765.1988.tb01421.x. [DOI] [PubMed] [Google Scholar]
  8. Hammarström L., Smith C. I. IgG subclasses in bacterial infections. Monogr Allergy. 1986;19:122–133. [PubMed] [Google Scholar]
  9. Kornman K. S., Robertson P. B. Clinical and microbiological evaluation of therapy for juvenile periodontitis. J Periodontol. 1985 Aug;56(8):443–446. doi: 10.1902/jop.1985.56.8.443. [DOI] [PubMed] [Google Scholar]
  10. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  11. Listgarten M. A., Lai C. H., Evian C. I. Comparative antibody titers to Actinobacillus actinomycetemcomitans in juvenile periodontitis, chronic periodontitis and periodontally healthy subjects. J Clin Periodontol. 1981 Jun;8(3):155–164. doi: 10.1111/j.1600-051x.1981.tb02027.x. [DOI] [PubMed] [Google Scholar]
  12. Mandell R. L., Ebersole J. L., Socransky S. S. Clinical immunologic and microbiologic features of active disease sites in juvenile periodontitis. J Clin Periodontol. 1987 Oct;14(9):534–540. doi: 10.1111/j.1600-051x.1987.tb00996.x. [DOI] [PubMed] [Google Scholar]
  13. Moore W. E. Microbiology of periodontal disease. J Periodontal Res. 1987 Sep;22(5):335–341. doi: 10.1111/j.1600-0765.1987.tb01595.x. [DOI] [PubMed] [Google Scholar]
  14. Ogawa T., Kusumoto Y., Hamada S., McGhee J. R., Kiyono H. Bacteroides gingivalis-specific serum IgG and IgA subclass antibodies in periodontal diseases. Clin Exp Immunol. 1990 Nov;82(2):318–325. doi: 10.1111/j.1365-2249.1990.tb05446.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Ranney R. R., Yanni N. R., Burmeister J. A., Tew J. G. Relationship between attachment loss and precipitating serum antibody to Actinobacillus actinomycetemcomitans in adolescents and young adults having severe periodontal destruction. J Periodontol. 1982 Jan;53(1):1–7. doi: 10.1902/jop.1982.53.1.1. [DOI] [PubMed] [Google Scholar]
  16. Schenck K. IgG, IgA and IgM serum antibodies against lipopolysaccharide from Bacteroides gingivalis in periodontal health and disease. J Periodontal Res. 1985 Jul;20(4):368–377. doi: 10.1111/j.1600-0765.1985.tb00448.x. [DOI] [PubMed] [Google Scholar]
  17. Schenck K., Michaelsen T. E. IgG subclass distribution of serum antibodies against lipopolysaccharide from Bacteroides gingivalis in periodontal health and disease. Acta Pathol Microbiol Immunol Scand C. 1987 Apr;95(2):41–46. doi: 10.1111/j.1699-0463.1987.tb00007.x. [DOI] [PubMed] [Google Scholar]
  18. Scott M. G., Shackelford P. G., Briles D. E., Nahm M. H. Human IgG subclasses and their relation to carbohydrate antigen immunocompetence. Diagn Clin Immunol. 1988;5(5):241–248. [PubMed] [Google Scholar]
  19. Sims T. J., Moncla B. J., Darveau R. P., Page R. C. Antigens of Actinobacillus actinomycetemcomitans recognized by patients with juvenile periodontitis and periodontally normal subjects. Infect Immun. 1991 Mar;59(3):913–924. doi: 10.1128/iai.59.3.913-924.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Spiegelberg H. L. Biological activities of immunoglobulins of different classes and subclasses. Adv Immunol. 1974;19(0):259–294. doi: 10.1016/s0065-2776(08)60254-0. [DOI] [PubMed] [Google Scholar]
  21. Tew J. G., Marshall D. R., Burmeister J. A., Ranney R. R. Relationship between gingival crevicular fluid and serum antibody titers in young adults with generalized and localized periodontitis. Infect Immun. 1985 Sep;49(3):487–493. doi: 10.1128/iai.49.3.487-493.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Tew J. G., Marshall D. R., Moore W. E., Best A. M., Palcanis K. G., Ranney R. R. Serum antibody reactive with predominant organisms in the subgingival flora of young adults with generalized severe periodontitis. Infect Immun. 1985 May;48(2):303–311. doi: 10.1128/iai.48.2.303-311.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Vincent J. W., Suzuki J. B., Falkler W. A., Jr, Cornett W. C. Reaction of human sera from juvenile periodontitis, rapidly progressive periodontitis, and adult periodontitis patients with selected periodontopathogens. J Periodontol. 1985 Aug;56(8):464–469. doi: 10.1902/jop.1985.56.8.464. [DOI] [PubMed] [Google Scholar]
  24. Wilson M. E., Hamilton R. G. Immunoglobulin G subclass response of localized juvenile periodontitis patients to Actinobacillus actinomycetemcomitans Y4 lipopolysaccharide. Infect Immun. 1992 May;60(5):1806–1812. doi: 10.1128/iai.60.5.1806-1812.1992. [DOI] [PMC free article] [PubMed] [Google Scholar]
  25. Wilson M. E., Schifferle R. E. Evidence that the serotype b antigenic determinant of Actinobacillus actinomycetemcomitans Y4 resides in the polysaccharide moiety of lipopolysaccharide. Infect Immun. 1991 Apr;59(4):1544–1551. doi: 10.1128/iai.59.4.1544-1551.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]
  26. Zambon J. J. Actinobacillus actinomycetemcomitans in human periodontal disease. J Clin Periodontol. 1985 Jan;12(1):1–20. doi: 10.1111/j.1600-051x.1985.tb01348.x. [DOI] [PubMed] [Google Scholar]

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