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. 1993 Apr;61(4):1547–1552. doi: 10.1128/iai.61.4.1547-1552.1993

Identification of a salivary agglutinin-binding domain within cell surface adhesin P1 of Streptococcus mutans.

P J Crowley 1, L J Brady 1, D A Piacentini 1, A S Bleiweis 1
PMCID: PMC281399  PMID: 8454362

Abstract

DNA encoding the alanine-rich region (A-region) of the cell surface adhesin, P1, from Streptococcus mutans was subcloned and expressed as a fusion protein with the maltose-binding protein (MBP) of Escherichia coli. The A-region fusion protein was shown to competitively inhibit both adherence of S. mutans to salivary agglutinin-coated hydroxyapatite and fluid-phase agglutinin-mediated aggregation of this organism. MBP alone or an MBP-paramyosin fusion protein was not inhibitory. Proteolytic cleavage of the fusion protein into its component moieties, MBP and A-region, resulted in breakdown of the A-region into three main fragments. Western immunoblot analysis of calcium-dependent agglutinin binding to this preparation revealed binding specificity for a 28-kDa fragment. Thus, the A-region of P1 is an important domain which interacts directly with salivary agglutinin, and this interaction interferes with both the aggregation and the adherence mechanisms in vitro.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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