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. 1988 Oct;85(19):7322–7326. doi: 10.1073/pnas.85.19.7322

Characterization of the cDNA encoding a protein binding to the major histocompatibility complex class II Y box.

D K Didier 1, J Schiffenbauer 1, S L Woulfe 1, M Zacheis 1, B D Schwartz 1
PMCID: PMC282178  PMID: 3174636

Abstract

The expression of HLA class II genes is regulated by a series of cis-acting elements and trans-acting factors. Several cis-acting elements have been identified and have been termed the Z box, X box, Y box, octamer, and "TATA" box. The Y box contains an inverted CCAAT box. By probing a phage lambda gt11 library with double-stranded oligonucleotides, we have directly isolated a cDNA encoding a Y box-binding protein designated YB-1. YB-1 binding has an absolute requirement for the CCAAT box and relative specificity for the Y box. It has a Mr of 35,414, contains 18% basic residues, and contains putative nuclear localization signals. An inverse correlation of YB-1 and HLA-DR beta chain mRNA levels suggests that YB-1 is a negative regulatory factor.

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