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Proceedings of the National Academy of Sciences of the United States of America logoLink to Proceedings of the National Academy of Sciences of the United States of America
. 1988 Nov;85(22):8439–8443. doi: 10.1073/pnas.85.22.8439

Cloning and sequence analysis of cDNA for corticotropin-releasing factor precursor from the teleost fish Catostomus commersoni.

Y Okawara 1, S D Morley 1, L O Burzio 1, H Zwiers 1, K Lederis 1, D Richter 1
PMCID: PMC282473  PMID: 3186733

Abstract

The sequence of a cDNA encoding the corticotropin-releasing factor precursor has been identified by screening lambda gt11 libraries constructed from poly(A)+ RNA of the hypothalamic region of the white sucker Catostomus commersoni brain with synthetic oligonucleotide probes deduced from the sequence of the rat corticotropin-releasing factor. The amino acid sequence of corticotropin-releasing factor of the sucker is strikingly conserved when compared to its counterpart from rat and differs only in two positions at the carboxyl terminus; in contrast, there is little similarity between their cryptic regions.

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Selected References

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