Skip to main content
PMC home page
Proceedings of the National Academy of Sciences of the United States of America logoLink to Proceedings of the National Academy of Sciences of the United States of America
. 1988 Dec;85(24):9499–9502. doi: 10.1073/pnas.85.24.9499

Structure and regulation of the rat 1,25-dihydroxyvitamin D3 receptor.

J K Burmester 1, R J Wiese 1, N Maeda 1, H F DeLuca 1
PMCID: PMC282780  PMID: 2849110

Abstract

Complementary DNA clones encoding the entire rat 1,25-dihydroxyvitamin D3 receptor were isolated, and the total 423-amino acid sequence was deduced. The amino acid sequence of the rat receptor is similar but not identical to the reported human receptor sequence. The cysteine-rich DNA-binding domain is completely conserved and the steroid-binding domain is greater than 93% conserved between rat and human. The cDNA was used as a probe in blot analysis of polyadenylylated RNA to show that the 1,25-dihydroxyvitamin D3 receptor mRNA is a single 4.4-kilobase mRNA that is expressed in intestine and kidney, slightly expressed in heart, and not detectable in liver and spleen. The receptor mRNA concentration is markedly increased during development of the rat intestine between day 14 and day 21, in accord with previous results obtained by measurement of receptor concentration by ligand binding or immunoblotting.

Full text

PDF
9499

Images in this article

9501Image
on p.9501
9501Image
on p.9501

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Baker A. R., McDonnell D. P., Hughes M., Crisp T. M., Mangelsdorf D. J., Haussler M. R., Pike J. W., Shine J., O'Malley B. W. Cloning and expression of full-length cDNA encoding human vitamin D receptor. Proc Natl Acad Sci U S A. 1988 May;85(10):3294–3298. doi: 10.1073/pnas.85.10.3294. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Brooks M. H., Bell N. H., Love L., Stern P. H., Orfei E., Queener S. F., Hamstra A. J., DeLuca H. F. Vitamin-D-dependent rickets type II. Resistance of target organs to 1,25-dihydroxyvitamin D. N Engl J Med. 1978 May 4;298(18):996–999. doi: 10.1056/NEJM197805042981804. [DOI] [PubMed] [Google Scholar]
  3. Brown T. A., Prahl J. M., DeLuca H. F. Partial amino acid sequence of porcine 1,25-dihydroxyvitamin D3 receptor isolated by immunoaffinity chromatography. Proc Natl Acad Sci U S A. 1988 Apr;85(8):2454–2458. doi: 10.1073/pnas.85.8.2454. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Burmester J. K., Maeda N., DeLuca H. F. Isolation and expression of rat 1,25-dihydroxyvitamin D3 receptor cDNA. Proc Natl Acad Sci U S A. 1988 Feb;85(4):1005–1009. doi: 10.1073/pnas.85.4.1005. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Dame M. C., Pierce E. A., DeLuca H. F. Identification of the porcine intestinal 1,25-dihydroxyvitamin D3 receptor on sodium dodecyl sulfate/polyacrylamide gels by renaturation and immunoblotting. Proc Natl Acad Sci U S A. 1985 Dec;82(23):7825–7829. doi: 10.1073/pnas.82.23.7825. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Dame M. C., Pierce E. A., Prahl J. M., Hayes C. E., DeLuca H. F. Monoclonal antibodies to the porcine intestinal receptor for 1,25-dihydroxyvitamin D3: interaction with distinct receptor domains. Biochemistry. 1986 Aug 12;25(16):4523–4534. doi: 10.1021/bi00364a011. [DOI] [PubMed] [Google Scholar]
  7. Darwish H. M., Krisinger J., Strom M., DeLuca H. F. Molecular cloning of the cDNA and chromosomal gene for vitamin D-dependent calcium-binding protein of rat intestine. Proc Natl Acad Sci U S A. 1987 Sep;84(17):6108–6111. doi: 10.1073/pnas.84.17.6108. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. DeLuca H. F., Schnoes H. K. Vitamin D: recent advances. Annu Rev Biochem. 1983;52:411–439. doi: 10.1146/annurev.bi.52.070183.002211. [DOI] [PubMed] [Google Scholar]
  9. DeLuca H. F. The vitamin D story: a collaborative effort of basic science and clinical medicine. FASEB J. 1988 Mar 1;2(3):224–236. [PubMed] [Google Scholar]
  10. Eil C., Liberman U. A., Rosen J. F., Marx S. J. A cellular defect in hereditary vitamin-D-dependent rickets type II: defective nuclear uptake of 1,25-dihydroxyvitamin D in cultured skin fibroblasts. N Engl J Med. 1981 Jun 25;304(26):1588–1591. doi: 10.1056/NEJM198106253042608. [DOI] [PubMed] [Google Scholar]
  11. Gubler U., Hoffman B. J. A simple and very efficient method for generating cDNA libraries. Gene. 1983 Nov;25(2-3):263–269. doi: 10.1016/0378-1119(83)90230-5. [DOI] [PubMed] [Google Scholar]
  12. Halloran B. P., DeLuca H. F. Appearance of the intestinal cytosolic receptor for 1,25-dihydroxyvitamin D3 during neonatal development in the rat. J Biol Chem. 1981 Jul 25;256(14):7338–7342. [PubMed] [Google Scholar]
  13. Kessler M. A., Lamm L., Jarnagin K., DeLuca H. F. 1,25-Dihydroxyvitamin D3-stimulated mRNAs in rat small intestine. Arch Biochem Biophys. 1986 Dec;251(2):403–412. doi: 10.1016/0003-9861(86)90346-2. [DOI] [PubMed] [Google Scholar]
  14. McDonnell D. P., Mangelsdorf D. J., Pike J. W., Haussler M. R., O'Malley B. W. Molecular cloning of complementary DNA encoding the avian receptor for vitamin D. Science. 1987 Mar 6;235(4793):1214–1217. doi: 10.1126/science.3029866. [DOI] [PubMed] [Google Scholar]
  15. Pierce E. A., Dame M. C., DeLuca H. F. Size and charge of the functional 1,25-dihydroxyvitamin D receptor in porcine intestine. J Biol Chem. 1987 Dec 15;262(35):17092–17099. [PubMed] [Google Scholar]
  16. Pierce E. A., DeLuca H. F. Regulation of the intestinal 1,25-dihydroxyvitamin D3 receptor during neonatal development in the rat. Arch Biochem Biophys. 1988 Mar;261(2):241–249. doi: 10.1016/0003-9861(88)90338-4. [DOI] [PubMed] [Google Scholar]
  17. Rosen J. F., Fleischman A. R., Finberg L., Hamstra A., DeLuca H. F. Rickets with alopecia: an inborn error of vitamin D metabolism. J Pediatr. 1979 May;94(5):729–735. doi: 10.1016/s0022-3476(79)80139-0. [DOI] [PubMed] [Google Scholar]
  18. Sanger F., Nicklen S., Coulson A. R. DNA sequencing with chain-terminating inhibitors. Proc Natl Acad Sci U S A. 1977 Dec;74(12):5463–5467. doi: 10.1073/pnas.74.12.5463. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Simpson R. U., DeLuca H. F. Purification of chicken intestinal receptor for 1 alpha, 25-dihydroxyvitamin D3 to apparent homogeneity. Proc Natl Acad Sci U S A. 1982 Jan;79(1):16–20. doi: 10.1073/pnas.79.1.16. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Proceedings of the National Academy of Sciences of the United States of America are provided here courtesy of National Academy of Sciences

RESOURCES